GrainGenes Probe Report: CDO1449

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Probe	CDO1449  [ Marker Report ]
Locus	Xcdo1449 cdo1449 cdo1449a
Synonym	Synonym HAR35_264   HAR35_49601
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Uni-ZAP XR library
Type	cDNA
Sequence	BE439316 BE439349 CDO1449_WHE05F06.s1
Polymorphism	CDO1449 EcoRI   CDO1449 EcoRV   CDO1449 DraI
Source Species	Avena sativa
Source Germplasm	Brooks CIav9260
Source Tissue	etiolated leaf
PCR size	1
Clone Vector	pBluSK
Excision Enzyme	EcoRI XhoI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E. GrainGenes Probe Repository
Clone Authority	Sorrells, Mark E.
Image	CDO1449 KO autoradiogram
Data Source	Sorrells, Mark E. 96.07