GrainGenes Probe Report: CDO524

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Probe	CDO524  [ Marker Report ]
Locus	CDO524 Xcdo524-6B Xcdo524
Reference	Heun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks	Uni-ZAP XR library
Type	cDNA
Sequence	AA231710 AA231938
Polymorphism	CDO524 EcoRI   CDO524 XbaI
Source Species	Avena sativa
Source Germplasm	Brooks CIav9260
Source Tissue	etiolated leaf
PCR size	1.2
Clone Vector	pBluSK
Excision Enzyme	EcoRI XhoI
Vector PCR primers	M13forward M13reverse
Vector Amplification	94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic	Ampicillin
Clone Location	Sorrells, Mark E.
Clone Authority	Sorrells, Mark E.
Image	CDO524 BROWS autoradiogram CDO524 SO autoradiogram
Data Source	Sorrells, Mark E. 96.07