GrainGenes Probe Report: EBmac405

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Probe	EBmac405  [ Marker Report ]
Locus	EBmac0405
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GT)16 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.21
Type	PCR SSR
PCR primers	5' ATGTAGCTCGGAATGTGTAGT 3' 5' CATGTTGGATAAGAGTAGAGGA 3' ATGTAGCTCGGAATGTGTAGT CATGTTGGATAAGAGTAGAGGA
SSR size	123
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare