| |
| |
|  | Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. |
| | Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. |
| | Saghai Maroof MA et al. (1994) Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proceedings of the National Academy of Science, USA 91:5466-5470. |
|
|
| | Insert contains repeat (AT)29. | | In 369 Avena sterilis accessions there were 3 alleles of size 138-142 bp, PIC = negative. |
|
| |
| 5' ACACCTTCCCAGGACAATCCATTG 3'
5' AGCACGCAGAGCACCGAAAAAGTC 3' | ACACCTTCCCAGGACAATCCATTG
AGCACGCAGAGCACCGAAAAAGTC |
|
| |
| | PCR comprised one cycle of 94oC for 3 min, 55oC for 2 min, and 72oC for 1.5 min, followed by 30 cycles of 94oC for 1 min, 55oC for 2 min, and 72oC for 1.5 min. |
|
| |
| |
| |
| |
| |
| | Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10. |
|
| |
| |
| |
