GrainGenes Probe Report: WMC213

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Probe	WMC213  [ Marker Report ]
Locus	Xwmc213f Xwmc213-2B Xwmc213-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3c6 Donor: Jack, Plant Breeding International No trimming done to insert. (CA)13 36 to 61, (CA)10 88 to 107. Bases 98-195 (ie 3' side of microsatellite) match (weakly) part of 2h12. Reverse primer here is within well matched region.
Type	PCR SSR
PCR primers	Forward ATTTTcTcAAAcAcAccccg at 8 length 20 bases, Tm 62 Reverse TAgcAgATgTTgAcAATggA at 172 length 20 bases, Tm 57
SSR size	184 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC213
Source Species	Triticum aestivum
Data Source	Somers, Daryl J. 2004.06 Isaac, Peter G. 2004.06