GrainGenes Probe Report: WMC283

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Probe	WMC283  [ Marker Report ]
Locus	Xwmc283.2-4A [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13d1 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 157 bases located on 7A No sequence 14.7.97. Resequenced. Trimmed to insert at both ends. (CA)19 64 to 101, (CA)8 104 to 119.
Type	PCR SSR
PCR primers	Forward cgTTggcTgggTTATATcATcT at 2 length 22 bases, Tm 62 Reverse gAcccgcgTgTAAgTgATAggA at 131 length 22 bases, Tm 65
SSR size	151 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC283
Source Species	Triticum aestivum
Data Source	Somers, Daryl J. 2004.06 Isaac, Peter G. 2004.06