GrainGenes Probe Report: WMC503

[ Printable Version ]  [ Submit comment/correction ]

Probe	WMC503  [ Marker Report ]
Locus	Xwmc503-2D Xwmc503
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20b8 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 237 bases located on 2D, 5B Trimmed to insert at both ends. (GT)11 112 to 133, (GT)18 192 to 227.
Type	PCR SSR
PCR primers	Forward gcAATAgTTcccgcAAgAAAAg at 55 length 22 bases, Tm 64 Reverse ATcAAcTAccTccAgATcccgT at 270 length 22 bases, Tm 63
SSR size	237 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC503
Source Species	Triticum aestivum
Data Source	Somers, Daryl J. 2004.06 Isaac, Peter G. 2004.06