GrainGenes Probe Report: WMS473

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Probe	WMS473  [ Marker Report ]
Locus	Xgwm473-2A Xgwm473 Xgwm473-7D Xgwm473-7A
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)14(TTGG)(GT)8 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCATACGGGTATGGTTGGAC 3' 5' CACCCCCTTGTTGGTCAC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS473
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring
Data Source	Roeder, Marion S. 98.09.15