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| | Identification of microsatellite polymorphisms in an expressed portion of the rye genome. |
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| | For genetic and applied purposes the number of SSR markers in rye has to be significantly increased to cover the entire genome. To this end, more than 8000 publicly accessible rye cDNA sequences from anthers, cold-stressed leaves, and aluminum-stressed as well as unstressed roots were exploited as a resource for SSR marker development. One hundred and fifty-seven Secale cereale microsatellite (SCM) loci out of 528 simple sequence repeats comprising di-, tri- and tetra-nucleotide motifs could be assayed on automated sequencers. One hundred EST-derived SCM loci displayed a length polymorphism among a sample of 15 rye accessions. Forty-five percent of the SCM could be associated with proteins of known or unknown function. As a conclusion, recently published ESTs from different rye tissues proved to be a valuable resource for SSR marker development in rye. |
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