GrainGenes Home

 
Query (optional)   in Class 

GrainGenes Sequence Report: BE493701

[ Printable Version ]  [ Submit comment/correction ]

Sequence
BE493701
Contig
Ta.28704.1.S1_at
NSFT03P2_Contig17517
Tracefile
[ Download ]
[ View ]
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Germplasm
T. monococcum ssp. monococcum DV92
Species
Triticum monococcum
Cultivar
DV92
Clone Library
Triticum monococcum vegetative apex cDNA library
Tissue
Vegetative shoot apex
Developmental Stage
Three weeks-old plants
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0574_C12_F24ZE Triticum monococcum vegetative apex cDNA library Triticum monococcum cDNA clone WHE0574_C12_F24, mRNA sequence.
Other Name
WHE0574_C12_F24ZE  [ wEST-ACEDB ]  [ wEST-mySQL ]
Strain
lab_host E. coli XLOLR
DNA Library
TM011XXX
Clone
WHE0574_C12_F24
Remark
DB_xref: taxon:4568
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene T3 primer.
Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda pBK-CMV (Lambda Zap Express), excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ggcacgagatggcgccgacctccaaatggcgctgggcatcaagcgcgcgt
cgcggtcgcacacctaccaccgccgcgggctgtgggccatcaaggccaag
cacggcggagccttccccaaggccgagaagccagccgccgtcgccgagcc
caagttctaccccgccgacgacgtcaagccccgcaccgccagcacccgca
agcctaagcccaccaagctcaggtcgaccatcacgcccggtacggtgctg
atcctgctcgctgggaggtacatggggaagcgcgtggtgttcctcaagca
gctcaagtctggcctgctcctcatctctgggcctttcaagatcaatggtg
tgccagttcgccgggtgaaccaggcttatgtcattgccacgtccacaaag
gttgacatctctggtgttaaggtggagaagtttgatgacaagtacttcgc
cagggacaagaagaccagggcaaagaagactgaaggggagctgtttgaga
ctgagaaggagaccaccaagaatctgccagaatttaaaaaagatgatcag
aaggccatcgatgctgagttgatcaaagctattgaggctgtctctgacct
taagaactatcttggtgctcggttctctctcagggatggtgacaagcccc
atgagatgaccttctaagttgctacaagttcctg
BLAST
BLAST Search

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.