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| | Locus Source: Hordeum vulgare subsp. vulgare | | UniGene title 'Transcribed locus, strongly similar to NP_001044831.1 Os01g0853400 [Oryza sativa (japonica cultivar-group)]' |
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| | germinating seeds, 16-48 h |
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| | genbank | Release 135, Apr 15 2003 | | genbank | Updated Nov 2006 |
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| | HU03J13r HU Hordeum vulgare subsp. vulgare cDNA clone HU03J13 5-PRIME, mRNA sequence. |
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| | lab_host XL10-Gold | | vulgare |
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| | DB_xref: taxon:112509 | | Feature: source: mol_type = 'mRNA'; | | Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 601 Std Error: 0.00; Plate: 3 row: J column: 13; Seq primer: M13rev. | | Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); germinating seeds, 16-48h. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1.2 kb |
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| ggggcacgagggccgcatgaccgtcgccgacgacgacgtcgccgcgctca
tccgctcccgcggccacatgctgcaggagctcaagctcgacaagtgctcc
ggattctccactgacgcgctccgcctcgtcgcacgctcctgcagatccct
gagaacattatttcttgaggaatgtgtgattactgatgaaggtggtgaat
ggcttcatgaacttgctgtcaacaattctgttcttgtgacactgaacttc
tacatgactgagctcaaagtggcgccggctgatctggagcttctagcaaa
gaactgcaaatcattactgtctttaaagatcagtgagtgtgatctttcag
acctgattggttttttcgaagcagccagtgcattgcaagattttgctgga
ggagcattcaacgaggtaggagagctaacaaagtatgaaaaagtcaagtt
tccaccaagagtatgcttcttggggcttacattcatggggaaaaatgaga
tgcctgtcatcttcccattttctgcttcactaaagaagctggacttgcag
tacactttccctcaccaccgaggatcattgccagcttatctcaaaatgcc
c
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