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| | Locus Source: Hordeum vulgare | | UniGene title 'CDNA clone: FLbaf102m02, mRNA sequence' |
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| | callus (5-10 mm in diameter) |
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| | genbank | Release 135, Apr 15 2003 | | genbank | Updated Nov 2006 |
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| | HD05N23r HD Hordeum vulgare cDNA clone HD05N23 5-PRIME, mRNA sequence. |
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| | DB_xref: taxon:4513 | | Feature: source: mol_type = 'mRNA'; | | Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 593 Std Error: 0.00; Plate: 5 row: N column: 23; Seq primer: M13rev. | | Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb |
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| agaagaggaatcgaccatggcgacccgtatccagtttgagaacaactgtg
aagttggtgttttctccagctacaaatgcctactgtctggttgcaattgg
aggatcagagaatttctacagtacatttgagtctgagcttgcagatgtca
ttcctgtggtcaagacctctatcggtggcactagaataattggccgcctc
tgtgttggaaacaagaatggacttctcttgccacacacaaccactgatca
agagcttcagcatctgaggaactgcatacctgatcaagtggttgttcagc
gcatcgatgaaaggctgtctgcccttggcaattgcatatcctgcaatgac
catgttgcgctcacacaccctgaccttgacaaggcaactgaggagcttat
tgcagatgtgcttggggttgaggtattccgtcagacaattgcaggaaata
tccttgttgggagctactgtgcattctctaacaggggtggactggtccat
cctcacacatccattgaagatcttgatgagctgtccacactcctccaagt
ccctcttgtcgctggaaccgtgaaccgtggtagcgaggtcatt
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