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| | Locus Source: Hordeum vulgare |
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| | genbank | Release 135, Apr 15 2003 |
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| | 2P-PCR-I Hordeum vulgare Wang&Wei Hordeum vulgare STS genomic, sequence tagged site. |
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| | DB_xref: taxon:4513 | | Feature: source: mol_type = 'genomic DNA'; | | Locus Comment: Contact: Richard Wang; Forage and Range Research Laboratory; USDA-ARS, Utah State University; 695 N 1100 E, Logan, UT 84322-6300, USA; Email: rrcwang@cc.usu.edu; Primer A: ACAATCTGAAAATCTGGACA; Primer B: TCATATTGAGACTCCTATAA; STS size: 249; PCR Profile:; Presoak: 0 degrees C for 0.00 minute(s); Denaturation: 93 degrees C for 1.00 minute(s); Annealing: 52 degrees C for 1.00 minute(s); Polymerization: 71 degrees C for 2.00 minute(s); PCR Cycles: 28; Thermal Cycler: Perkin Elmer 9600; Protocol:; Template: 40 ng; Primer: each 3 uM; dNTPs: each 200 uM; Taq Polymerase: 0.04 units/ul; Total Vol: 25 ul; Buffer:; MgCl2: 2.5 mM; KCl: 50 mM; Tris-HCl: 10 mM; pH: 8.3. | | Note: V-type: Unknown |
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| | primer_bind | 1 | 20 | | Location: 1..20 | | STS | 1 | 249 | | Location: 1..>249 |
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| acaatctgaaaatctggacagaatttgcaaatggatgttgtgctcaaact
ttgaactgtggaacttggattttgatggaaatggtgaatatatggatatg
agaagctccacaaattatttgggatttttcctggctacccaaatggtagt
tcctttagaaactgccccaaatgcaatattggcattaaataggaaaaggc
aattccccccataaaaaatggcccattttttattggagtctcaatatga
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