GrainGenes Trait Study Report: DON accumulation, Somers03

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Trait Study	DON accumulation, Somers03
Trait	Deoxynivalenol (DON) accumulation
Reference	Somers DJ et al. (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat Genome 46:555-564.
Protocol	Plants harvested at maturity and a 1g sample of seed was milled and analyzed for DON by an ELISA method
Values	Levels of DON accumulation were measured in ppm.
Environment	Ottawa 1999 Ottawa 2000
Parental Description	MARINGA DON (ppm) = 3.5 for lines homozygous for MARINGA alleles (MM) at QDon.crc-3B; DON (ppm) = 3.8 for lines homozygous for MARINGA alleles (MM) at QDon.crc-5A WUHAN #1 DON (ppm) = 5.4 for lines homozygous for Wuhan alleles (WW) at QDon.crc-3B; DON (ppm) = 5.9 for lines homozygous for Wuhan alleles (WW) at QDon.crc-5A
Map Data	Wheat, WxM, FHB QTL
Population Size	91
Population Type	Doubled Haploid
QTL Analysis Method	ANOVA
Markers Tested	600
QTLs Found	2
Phenotypic R2	0.25 p < 0.001
QTL	QDon.crc-3B QDon.crc-5A