Using the BatchPrimer3 program we have designed thousands of primers in several genomic research projects, including conserved intron-spanning primer pairs from EST sequences for wheat SNP discovery, SNP genotyping primers for wheat SNP mapping, standard primer pairs from Brachypodium bacteria artificial chromosome (BAC) end sequences for Brachypodium SNP discovery, sequencing primers from EST sequences for gene-specific sequencing, and SSR flanking primer pairs from Brachypodium EST and BAC end sequences for Brachypodium SSR genotyping. Most of these primers have been validated in experiments from several laboratories..

1. Wheat conserved intron-spanning primer sets.
2. Wheat SNP genotyping primer set from 1527 genome-specific loci
   • Single base extension (SBE) primer sets
   • Allele-specific orimer sets
   • Tetra-primer sets for for tetra-primer ARMS PCR
3. Wheat sequencing primer design
   • Notes of sequencing primer desing using EST sequences
   • Sequencing primer list
4. Brachypodium SSR detection and SSR-flanking primer sets.