| Triticeae species of origin | Genotype or cultivar or accession of origin | Tissue | Developmental stage | Condition (environmental stress; disease challenge; etc.) | Vector | Size of library (est. no. of clones) | Status (prescreened?; any sequencing done? confirmation of insert presence? indication of insert size? etc.) | Contact (if library is available) | ITEC contact if source of ESTs | Comment |
| Libraries from which sequences will be contributed to ITEC | ||||||||||
| Aegilops speltoides | . | anthers | immature; at meiosis | normal conditions | . | . | . | . | Jan Dvorak | . |
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| Hordeum vulgare | Barke | leaf | 14 day | normal growth | pBS/SK- (Stratagene) | 5 million | prescreened for SSU, LHC, sequencing, 99% with insert, average insert size: 850 bp | . | Prof. R.G. Herrmann | . |
| Hordeum vulgare | Forester | inflorescence | immature; developing | . | Lambda Zap II (Stratagene) | greater than 1 million clones | good library + well used | Dave Laurie | Paul Bailey | . |
| Hordeum vulgare | Franka | epidermal cells | 10-day-old seedlings | normal conditions | Triplex 2 lambda | 1 million | being tested for insert size and sequencing, selecting only clones with insert size between 400 and 1,500kb. | Catherine Feuillet | Catherine Feuillet | Preliminary sequencing and BLAST analysis indicate good value for this library. |
| Hordeum vulgare | Nure (2-row; winter) | leaf | first leaf | cold stress | UNI-ZAP XR | 1 million clones | made February 1999, average insert size 1kb, range 0.7-2.0 kb; sequencing started. | Nicola Pecchioni, Valeria Terzi | Nicola Pecchioni | . |
| Hordeum vulgare | Sultan 5 | leaf | . | 6 and 12 h after infection with Erysiphe graminis f. sp. hordei | pBAD42 - Clontech | 1 million clones, 90% have insert | 1.4 kb | . | Pietro Piffanelli | . |
| Hordeum vulgare | Triumph | whole grain | mature | 3-day malted | Lambda Zap express | 40,000 | good insert size in over 500 sequenced | . | Gordon C. Machray | . |
| Hordeum vulgare | . | culm | . | etiolated | . | . | . | . | Andreas Graner | . |
| Hordeum vulgare | . | leaf | . | etiolated | . | . | . | . | Andreas Graner | . |
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| Lophopyrum elongatum | Accession D | root | seedling | salt-stressed with 250 mM NaCl for 6 hours under hydroponic conditions. | PBluescript SK-, converted from original cDNA library in Lambda zap | about 500,000 | 80-90% inserts with average size about 700 bp | P. Gulick | P. Gulick | Limited copies of the Lambda zap library are available. |
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| Secale cereale | Blanco | root tip | young roots | stressed for aluminum | in the process | not yet known | not yet | Perry Gustafson | Perry Gustafson | . |
| Secale cereale | Blanco | root tip | young roots | nonstressed for aluminum | in the process | not yet known | not yet | Perry Gustafson | Perry Gustafson | . |
| . | . | . | . | . | . | . | . | . | . | . |
| Triticum aestivum | Atlas (Al tolerant) | root | 8 day old | grown in vermiculite with 0.6 mM CaCl2 in water | pYES2 (Invitrogen - yeast expression plasmid vector) | 8.7x10 ^6 | size selected for 500 - 1500bp, not sure about frequency of inserts | . | Marilyn Warburton | . |
| Triticum aestivum | Bobwhite | endosperm | mature seed | nonstressed and insect-infected (Russian wheat aphid) | . | . | screening underway, insert size >500 | Anna-Maria Botha-Oberholster | Anna-Maria Botha-Oberholster | . |
| Triticum aestivum | Catoctin (USDA-ARS, Beltsville MD) | leaf | four fully developed leaves after seed germination | normal greenhouse conditions | pSport-P | Primary library 1.12x10^7 cfu/ml | in progress | J.-J. Lin | J.-J. Lin | Contact Dr. Lin for information about the library. With sufficient interest, it could become an LTI product. |
| Triticum aestivum | Cheyenne | endosperm | 5 - 30 days post anthesis | control | lambda-Zap II | 1,000,000 | initial sequencing without prescreening - later sequencing after some normalization; about 50% of cDNAs of coding regions of 2000-2500 bp are full-length. | Olin Anderson | Olin Anderson | . |
| Triticum aestivum | Chinese Spring | anther | early meiosis | . | . | . | . | . | Peter Langridge | . |
| Triticum aestivum | Chinese Spring | root | . | . | . | . | made in 1995, well used | Dave Lonsdale | Paul Bailey | . |
| Triticum aestivum | Chinese Spring | scutelum callus | undifferentiated | . | . | . | made in 1995, well used | Dave Lonsdale | Paul Bailey | . |
| Triticum aestivum | Chinese Spring | spikelet | immature, developing | . | . | . | in preparation | Graham Moore | Paul Bailey | . |
| Triticum aestivum | Chinese Spring | spikes | immature | . | pT-Adv | 100 | cDNAs are differential display products. Sizes of inserts are known and in some cases have been assigned to chromosomes. | . | Bikram Gill | . |
| Triticum aestivum | Gamtoos-R (1RL.1BL translocation) | leaf & stem | 12- to 15-day-old seedlings | infected with Russian Wheat Aphid | pCMV Sport 6.0 | not complete, est. at 3 million | . | . | Nora Lapitan, Dennis Knudson |
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| Triticum aestivum | Germplasm line P29 | leaf & stem | 9-day-old seedlings | unstressed | Lambda Zap Express | 1 million | average insert size is 0.9 kb with 85% of clones having an insert | . | Joseph M. Anderson | . |
| Triticum aestivum | Mustang | leaf | 10-day-old seedlings | heat stress | Lambda Zap II (Stratagene) | 2 million | five full-length HSP clones isolated and sequenced (clone lengths of ca. 1 kb and ca. 3kb) | Henry Nguyen | Henry Nguyen | Could produce cDNA libraries of other conditions and genotypes. |
| Triticum aestivum | Norin 26 | spikelet | Feeke's scale 6 | field grown | . | 782,000 fpu | nonscreened, confirmed the insert, average insert size ca. 1.2kbp | Yasunari Ogihara | Yasunari Ogihara | Norin 26 is spring-sown Japanese cv., no vernalization requirement, early flowering, shows photoperiod cytoplasmic male sterility in combination with cytoplasm of Aegilops crassa. |
| Triticum aestivum | Novosibirskaya 67, red grain isoline | pericarp/testa | 3:1 mix, respectively of 10+20 days post anthesis | . | Lambda Zap II (Stratagene) | 2 million | made Dec. 1998 | Paul Bailey, John Flintham | Paul Bailey | . |
| Triticum aestivum | Odeon | root | seedling | unstressed | pUC18 | 819 | screened from original library of 2104 clones by size, which inserts ranging from 0.3 to 2kb, most around 1 kb. | J.M. Jacquemin | J.M. Jacquemin | 29 clones from this library have been mapped to the Opata x Synthetic wheat reference population (clones gbx). |
| Triticum aestivum | Palmiet | leaf | 4-leaf stage | nonstressed and insect-infected (Russian wheat aphid) | . | . | screening underway, insert size >500 | Anna-Maria Botha-Oberholster | Anna-Maria Botha-Oberholster | . |
| Triticum aestivum | Soleil | endosperm | 1:1 mix of 10+20 days post anthesis | . | Lambda Zap II (Stratagene) | 2 million | made Dec. 1998 | Kay Denyer | Paul Bailey | . |
| Triticum aestivum | Sumai #3 | spikes | at anthesis | challenged with head blight fungus | pGemT | 200 | . | . | Bikram Gill | . |
| Triticum aestivum | TAM 101 | root | 10-day-old seedlings | water deficiency stress | Lambda Zap II (Stratagene) | 400,000 | cDNA clones isolated so far are full length. | Henry Nguyen | Henry Nguyen | Could produce cDNA libraries of other conditions and genotypes. |
| Triticum aestivum | Thatcher Lr1 | leaf | young leaf | 24 h after challenge with LR-AVR1 | Lambda Zap | 1 million | screened with resistance gene analogs; only three full sequences obtained; insert size est. 1 kb. | Sylvie Cloutier | Sylvie Cloutier | . |
| Triticum aestivum | Tugela | endosperm | mature seed | nonstressed and insect-infected (Russian wheat aphid) | . | . | screening underway, insert size >500 | Anna-Maria Botha-Oberholster | Anna-Maria Botha-Oberholster | . |
| Triticum aestivum | Wyuna | endosperm | mid-development | . | Lambda Zap | . | . | . | Rudi Appels | . |
| Triticum aestivum | . | embryo | . | . | . | . | . | . | Mike Holdsworth | . |
| Triticum aestivum | . | endosperm | . | . | . | . | . | . | Mike Holdsworth | . |
| Triticum aestivum | . | leaf | seedling | challenged with an avirulent isolate of wheat yellow rust | Lambda Zap Express (Stratagene) | . | in preparation | Lesley Boyd, Rob Koebner | Paul Bailey | . |
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| Triticum monococcum | DV92 | shoot apex and young leaves | 5-week-old plants | not induced for flowering | Phagemid pBK-CMV (4518 bp) Primers T3 and T7 | 764,000 | Two empty colonies from 24. Average size 1000 bp. 50 clones sequenced | . | Jorge Dubcovsky | . |
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| Triticum turgidum ssp. durum | Siliana | root | 3-day-old seedling | drought stress | pSPORT1 (BRL) | about 50,000 before amplification | screened for dehydrins; clones between 0.3 and 2 kb. | Philippe Joudrier | Philippe Joudrier | An additional 2000 sequences will be contributed, possibly from other libraries, during INRA's participation. |
| Libraries available; but use for ITEC sequences not yet confirmed. | ||||||||||
| Hordeum vulgare | Morex | shoot; etiolated | 5-day-old seedling | no stress; dark grown | Uni-ZAP XR vector; excised pBluescript phagemids; directionally | 6x10^5 pfu; mass excised to yield 1.3x10^6 cfu/ml on SOLR (Stratagene) cells; amplification=148; nonrecombinant clones=3%; blue colonies=5%; but 75% of these had inserts. | insert size ranges from 0.6 to 2.2 with peak at 1.6 kb; no sequencing done up to now. | Phagemids: Tim Close, Arrayed library (later): Rod Wing |
Andy Kleinhofs | We do not recommend sequencing from this library at this time. Once it is arrayed and the abundant mRNA clones removed; it will be an excellent source for random EST sequencing. |