INRA CLUB SSR-Screening
Varieties included in the screening :
line 1 ALTAR
- line 2 OPATA
- line 4 CHINESE SPRING
- line 12 SOISSON
- line 3 5 6 7 8 9 10 11 Syngenta germplasm
SSR Primer Pairs
|
Name |
Leftprimer |
Tm |
Rightprimer |
Tm |
|
gpw93012 |
TTGGGAAGAGGACCAGGG |
61.0 |
GCCATGAACTGCTGGGAG |
60.4 |
|
gpw93014 |
CCCCTGAGAACGAAAACAAA |
60.1 |
TGGGTCAACTATGTGTGGGA |
59.8 |
|
gpw93020 |
CTCTGCTTCGTGTCGCAGTA |
60.3 |
AGTTCATCGGTCGTTGCAG |
59.8 |
|
gpw95003 |
TCATTCGCTCATACGATAGGG |
60.1 |
CTGGATCACCTACTTTTCTCCG |
60.1 |
|
gpw95011 |
CCAAACAGCGACATTGAGC |
60.4 |
AGAGGTTGAAGACGGTGGG |
60.1 |
|
gpw95017 |
AAAGAAGAGGGGGGACCAC |
60.3 |
TTGTCAGCGGATTGGACTC |
59.8 |
|
gpw95019 |
ATTTGACGGACTGACAGCG |
59.8 |
CTGCTTCCAAGGATTGTTTTT |
58.4 |
|
gpw95023 |
TGAGCTCCACATCTCCAT |
63.0 |
CAACTCCTCTCTCACTCATC |
61.0 |
|
gpw95026 |
ATGGGCCTTTAAGAGCAAAA |
58.8 |
AGGGGTGAAAGGTTGGAGAC |
60.3 |
|
gpw95028 |
AGTATCGCAACGGCAACAG |
59.9 |
ATTTCACTCCCGCATTTGAC |
59.9 |
PCR protocol
· used in Clermont-Ferrand Tm : 60°C
PCR Profile :
5 min., 94°C
30 cycles 30 sec. 94°C
30 sec. Tm
30 sec. 72°C
10 min. 72°C
PCR Conditions
DNA 80 ng
dNTP 200 µM
primers 450 nM each
Taq 0.4 u
(PCR performed in 10 µL)
>gpw93012
TTTGGGAAGAGGACCAGGGCCTCTATAAATAGGATCAGCCACCCGCAAGACACATGGATG
ACGACGGACGACGACGACGACCACAGACACACACAGGGACAGAGAGCTGAGGGGTTCGGA
GAAGGAGGCTCTTAAGAGAGGGGTTTAGATCATATATATATATATATATATATATATATA
TATATATATATATATATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGGGTGAC
TGAGCTCCTCCCAGCAGTTCATGGCC
gpw93012
>gpw93014
GTCGCAGCGCCCCGCCGCCGGCCTGCTGCTGCATCCACTGCAGCTCGCCCCCGACTGGCT
GCCGGCGCCCACGCCACGGGCCGCGTCCGAGGCCAGCCCCGTTCGGCCTCGGGCACGGGC
ACCACGCCCGCACCCAGCGCCCGACCCGCAAGGCCTCTGTGCCACAGACAACGGGGCCCA
CCCCCTGAGAACGAAAACAAAAAAAATAATACTAATTAAATTAATTTTTTAAAAAATGAA
TTAATAAATAATAACTAAAATTAATTAAAATGATAATTAATTAATTGATTAAAGGCTTAA
TTAAATTAATTAGTCTTGATTAGATTAACCTAATTACTAGTTAACTTAATTAACCCCCTG
ATTAGACTAATCAATCAATGACATGCGGGTCCCACACATAGTTGACCCAGTCAACTCTGT
TGACTGCTGACGTCAGCATGACATCATGCTGATGTCATAAAACCAAATT
gpw93014
>gpw93020
AGGTGCTGTCGGGGTTGCAGTTCTGCGGGTACGACCCGTCGTCGCGGTTGGGCCACAGCC
CGTGGATCCCGAAGTCCGCCGCCGGCTTGCCGGACCGCGGGTAGCAGCAGCTCTGCTTCG
TGTCGCAGTACGACCCCGGCCACTGCACACACACACACACACACACCAAGGCCAACCGCC
GGCACGAACCATCCATCAGAAACCAAGCAGCACGTACACCACGTCTCGGCAACTGCAAGG
CTGCAACGACCGATGAACTGAACCGGCCACACGCGCACTCACCTGCAGCACGAGGTAGAA
GAAATCGTAGCCCGCATGTTTCCCGCTCTCCTGCGCTGCCGCGCAGCCGACGCTCAGGCC
GATCAGGAGGGCCAGGGAGCACTGCAACTTCATGGCTTTGGATGTCTTTGCTGGTTGTAT
TGATGGTTCTTCGGAGATGAGATGAGATGAGATGGAAGCTTCCGTTGGTGTTGCGGATTT
ATAGGCTGCGTTGATGTGGATCTACTCCACGGCAAATGCCAGGTTTCCGGAACGTGCATT
CCAAGGAGGATTTAATTGCTTGCACTTTTGGGCTTGCGGGAATGCCAGAGATGGAAGCAT
GGCTGTGGGTGGCGAGGTGTGCAAGCCAAGCAGCCGAACCAATTGTAGAATCCACGGGTC
CAGCCATAAAAAGATAGCATACACAACTACTAAAACATACAGAAATCACCAAGGAATAAA
GAGCTTTGCTACACATACATAATGTTACAGGGATGAGGGGTTTACTGGGTGTAGAGGCAC
AGGCCCACCCGCATTAAAATCAGGGAGATGTGTTTAGAATTTTTTTT
gpw93020
>gpw95003
GTGCAGCACATTTTTCACTTTCAGGTCTTTTTTTATCTGCAAAAGAAGAGAACATATTAG
AGCAGCTGGCTGTATAAGATTGCCGTGTCATCAAGAGGTACCAATATCATTCGCTCATAC
GATAGGGTTGGCTGGCTAGTGATATTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT
CTCTCTCTCTCTCTCTCTTTCTATTTCCTCTCATTTAACTAGGAGCACGTGAAGAGCTTG
GGCATTTGTTGCCTTCCACTATGTGGCCGATGCCATATTTCGCACCGCTCTCGCGATGTC
AGAGAGTTGGCACCGCTGTGCACACAGACCCACATGTATAAACAAATCAATTAAACCAAC
TACACCAGCCTCTCACTCTCCCAAACAAGTGTTTTTTTATTGCCTCAGTCCTCTCTCTCT
CCCACGCAGTGTTTTTTCATTGCGTGAATTAATTTGGCACCGGAGAAAAGTAGGTGATCC
AGATTGGGGCACCAGGTGAGCAATGGAGGGGC
gpw95003
>gpw95011
TCAACGTTCTTATGAGGTGCAACCTGGATCGTGAAGATTGTGTTAGTTTGCATTCCCCCT
TCATCTTACCCTACGCTTGAATCTCGGGACGAGATTCTTGTTTATTGGGGGTGACTTGTC
ACATCCCTAGCTTCTGGCAATGCACTAGGCTAGCCTCATGAGTGTCATCATATTTAAGTT
TCAAATGAACTTGAAATGGGGATTGTTTAAACCCTAGCATCAAAGGAATTCACTAGGTTC
AACTAAAATATTCTTAGTGAGTCCAAATGGCTTTCAAAAATGTTCATCGTTTCCGGAAAA
TGCTAGAAGCAATAACCAGAGGTGTTGCACATTTTTCCATGACATATTTGGGCTCTGAAT
TAAATCATATAGTATTTGCATTTGGGCATATAAATGCTATTAAATATTTTAAATGCTCAA
ATAACCATAAACTAAAATGTTTGCTGTTGGCTATATTCCAAACAGCGACATTGAGCAGTT
TCATGATTTTTGGATCTGTTTAAGTATTTTTAATAAAGCCCTAAAGACTACAGAATAATA
GAAAACAGAAACTAAACAGGAAATAAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACT
TACTGGCTTACCTGCGCAGCCCACCTGGCGGCCCAAGTGGCCAGCCCAGCCCACTGGCCT
GGCACTGTTGGCCAGCCCACCAGGAGCCCCACCGTCTTCAACCTCTGCTAGTAGGCAGAG
GCGT
gpw95011
>gpw95017
CAAAGAAGAGGGGGGACCACCCCGCTGATTGATAAGGCGCCCTCTTACTTATATACTACT
CTAGACTAAAAGTGTGCATGAGAGAGAGAGACAGAGGGAGAGAGAAAGAGAGGAGGGAGA
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACGAGAGATAGTGGAAAAAGAGTTGCA
ACATACAGTGCAAGTAAGAAAGTGTGCAGGGAAGAAAGGAAAGCGGAAAAAGAGAAGAGA
GAGAGAGAGAGAGAGTGAGAGAGAGAGAGAGAGAGAGAGTATAATATTCCACGAGTCCAA
TCCGCTGACAACTTTTAGTAATGTGACACCACGTCATCACCCGGTCATTATTTCTAACCG
TTTTTCGGCCTGTCGCT
gpw95017
>gpw95019
CCGGTTTGTTACGCGGTTTAAACGGGATTCGCCTGCTGTTAAATTTGACGGACTGACAGC
GCGGCGCGGCAGATAGAGATAGAGAGAGAGAGAGACTGCCGCGCGGTTTAAACGGAATCA
CTTGCTGTTAATTTGACAGCTAGCGTGGCGCAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
GAGAGAGAGAGAGAGAGAGAGAGAGTTGTACAGAAAAAAAACAATCCTTGGAAGCAGATC
TCAACCGGATCTG
gpw95019
>gpw95023
ATGAGCTCCACATCTCCATGGATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGAGGAGGA
GGAGGAGGAATATGTATTGCAGTACAACAACTTTCCATCTTTTTCCAAAATGAGCTCCAC
ATCTTCATGGATGAGTTCTATCAAGATATGCACATGAACTAAGTCGCAAGAACTAGAAGC
AAGATGAGTGAGAGAGGAGTTGCCGGATGCCTATGTTTGAGTTGTTCTTGACAACACTCT
TTGGCCCCTAAGTAATGGCGTTGCGCCAGCCAAAAGGTGTGGCTATGGTTGAACACAACC
AAGCCCCCAAGTGACTTACTCTCGTGGCTTTTGAGCCAGCCAAGAGGTGTAGCTATGGT
gpw95023
>gpw95026
ATCAGAGATTAGTATTACTACTGGTCAAGAGATTAGGTACTAATGGGCCTTTAAGAGCAA
AATAAATGAAAATGACTCACCCTAAAATCAGGCCAACCTCTCTCTCTCTCTCTCTCTCTC
TCTCTCTCACACACACACACACACACATTCACTGCATGGTTCTTAGTAGAGCGATCCGAT
GGCATGTGCTCCCATTCATGGTCTCCAACCTTTCACCCCTCTAATTAGTTTCTACTATGA
TAATATTTGTTCTTTCTGATACTTCATTAATATTTTCTTGTTGGGTAGATTTACATTTTA
AGATAAAATGTGAATTCACTGAAAAAATCTGTAGCATCAGGATAATTTATACAAATATTT
TAATTTATATTTTTTAAATAAAGGGCGTAATTTTCATTTTGCACGGGGCCCCAAATTTTA
AAGGTACGGCCCTGATCAGTCACATCAGAATTGGAATGTAAGTTAGTTTCACAGTGATGT
TGATTGAAGGATCATCCAATAATGCAAAGTTCCTTTTTGTCTGCAAACTTATTTGCTTCT
TAAAAAGAAAATAATTGTGTTTTTTTACCTCACCTCTCCCATCACAGAGAAAAAAAAAGA
CAATCCTTCCAAGGACTGGTTCAAACATTTTTTTTATCGGAATTTGCATCTAGGAGTAAT
AAATAAAAAGATAAGCAATTATATATTTCAAACTTAAAATTTGAGAGCCAGGTAGTTGG
gpw95026
>gpw95028
GCTCCACGGGAAGTGTAGCCGGGCTTAGGCTGCAACGCTCTGGAGTGGGGGATGGGGATG
GGGATGGGGATGGGGATCTGTGGGAAAAGGGGCGTTTGGCAGGCGTCATCTAAGAAACTC
AGGGCGGCCTTGGTATGGAGATCAGTGGGTAAGCTGCACGGGCAAACCGGCAGATGTTGA
CAATGGAAGTATCGCAACGGCAACAGCGGCGGCGGGGACCTTGCTAGGGTTTTGACCGAG
GGAGGGGGTGTGCGTGTGCACGCGCCCGAGGAGGTTTTAGTGTGTGTGTGTGTGTGTGGA
AGGGGGGGGTGTTTGAGGAAATGACGAGGGTACTAAATTTTTTTACTACGCGAGAGTCAA
ATGCGGGAGTGAAATGCCGGGGCTATTGAAAATTTTGATTATGTTGCATCGCACACGGTC
CGGAGATAACAACTGTGTGTTATGAAATAGAAAAACCCTTGAGGCAGGCATATATTTTCT
AGGGGCAGGTGGGATTGGGAACAAGAAACATCACACACGGTCCGGAGATAACAACCATGT
GTTATGAAACAGAAAAACCC
gpw95028