IV.9. Function and activity of Genetic and Aneuploid Stock Center*.
T. Tsuchiya. Department of Agronomy, Colorado State University, Fort Collins, Colorado 80521, U.S.A.
* The work reported in IV.9. (p.146 ) - IV 11. (p. 155) has been supported in part by NSF Research Grant GB4482X and GB 30493, and Colorado State University Experiment Station Project (Hatch 8).
The Genetic Stock Center in the Agronomy Department, Colorado State University, Fort Collins, Colorado, U.S.A., had been operated for a long time by the late Dr. D. W. Robertson. The Center's operation has been supported by a research grant from the National Science Foundation (NSF) of the United States of America since 1961 with the contract title "Assembly, Maintenance, Distribution, and Complementary Research Studies of Genetic and Chromosome Tester Stocks in Barley". The Stock Center is now operated by T. Tsuchiya with continuous support from National Science Foundation since 1969.
Since T. Tsuchiya, coordinator of research in trisomics and aneuploids, joined Colorado State University in 1968, trisomics and other aneploid stocks are included to the operation of the Center.
In Tables 1-4 is a listing of the number of genetic and trisomic stocks handled in the Center from 1969 to 1971.
The genetic stocks are periodically grown for increase and for restoration of viability. In 1969, 422 stocks were grown in the field. In 1970 and 1971, 302 and 401 stocks were grown in the greenhouse. Also, newly received stocks have been grown in the greenhouse in 1970 and 1971. The number of stocks grown are shown in Table 5.
Table 1. Genetic stocks sent out.
Table 2. Genetic Stocks Received.
Table 3. Trisomic and aneuploid stocks sent out.
Table 4. Trisomic stocks received.
Table 5. Number of genetic and aneuploid stocks grown in 1969-1971.
The total number of genetic stocks kept in the Stock Center is about 2000. In the past three years we have been working toward establishing a system for the maintenance and distribution of genetic and aneuploid stocks efficiently and effectively. About 1000 stocks have been received in the stock center in the past three years. The exact number of stocks will be reported in the next issue of BGN.
It was found that the Stock Center does not have many stocks for designated genes listed in the Summaries (1941, 1947, 1955, 1966), and the two symposium reports (1964, 1971) prepared by D. W. Robertson and co-authors and Reviews by L. Smith (1951) and R. A. Nilan (1964). Effort will be made to collect as many of these missing stocks as possible in the near future. Cooperation of barley research workers who have found new mutants and received gene symbols for them, yet have not sent seeds to the Stock Center, is appreciated. For old stocks used by some deceased research workers, we will ask research workers in the same country as those deceased researchers if certain stocks are still available. Special cooperation from these people will be highly appreciated.
The other areas of the activity of the Stock Center are (1) allelism testing among many similar type mutants in our stocks, (2) studies of aneuploids and (3) the establishment of multiple genetic marker stocks for each arm of each chromosome.
(1) A large number of mutants have been collected during the past 50 years in the Stock Center. Very little allelism testing had been done until recently. Some of the results of allelism tests are presented (Tsuchiya, 1971. BGN 1:62 and 1:65; Tsuchiya, 1972, BGN 2:80-87; McMullen and Haus, 1971 BGN1:68-69; MuMullen, 1972. BGN, 2:76). Additional allelism testing is now underway with various types of mutations. In this way, the number of stocks to be kept in the Stock Center can be reduced.
All the stocks which have been maintained in the Stock Center will be stored in the National Seed Storage Laboratory, U. S. Department of Agriculture, Fort Collins, Colorado, U.S.A. Only one genetic stock for each locus will be maintained in the Stock Center for distribution and genetic-linkage studies.
The complete list of barley genetic stocks in both the Stock Center in the Agronomy Department of Colorado State University and the National Seed Storage Laboratory will be published together with available information for the stock in the Barley Genetics Newsletter in the near future.
(2 ) It has been well demonstrated that genetic and linkage studies by means of trisomic analysis is a most effective and efficient method. The Barley Genetic Stock Center here in Fort Collins is also the center of stock maintenance and research with primary trisomics, teletrisomics and other aneuploids in barley. Primary trisomics and telotrisomics have been and are being used for associating new mutant genes with a chromosome (primary trisomic analysis) or with a chromosome arm (telotrisomic analysis) (Tsuchiya, 1971, BGN 1:61-62; 1972, BGN 2:93. In addition to this a large number of plants from 329 F1 combinations and 172 F2 populations were grown in 1969 to 1971. An extensive telotrisomic analysis is now underway in the Stock Center. A total of 47 marker genes on five chromosomes (1-5) have been or are being tested by means of telotrisomic analysis. At least 10 or more marker genes, most of which are lethal chlorophyll mutations, located or associated with the five non-satellited chromosomes will be studied by means of telotrisomic analysis. These results will give rather precisely the centromere position for each of those five chromosomes in the near future.
(3) Once the centromere position is located in the linkage maps in relation to the marker genes, then it will become easy to establish multiple marker stocks for each arm of, at least, the five nonsatellited chromosomes.
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