4. Protease Inhibitors

4.1. Trypsin inhibitors
 
Ti-H1. [Itc 1 {528}].   3H {528}. ad: CS/Betzes.
Ti-R1.   3R {529}. ad: CS/Imperial.
Ti-A2 {699}.   5AL {699}. v: CS.
Ti-B2 {699}.   5BL {699}. v: CS.
Ti-D2 {699}.   5DL {699}. v: CS.
Ti-D2a {699}.
    v: CS.
Ti-D2b {699}.
    v: Champlein.
Ti-D2c {699}.
    v: Synthetic.
Ti-Agi2 {699}.   5Agi {699}. ad: Vilmorin 27/ Ag. intermedium.
Ti-R2 {699}.   5RL {699}. ad: CS/Imperial.
su: CS/King II.
Ti-Mt2 {699}.   5Mt {699}. ad: CS/Ae. mutica.
Ti-Sl2 {699}.   5SlL {699}. ad: CS/Ae. sharonensis.
Ti-U2 {699}.   1U {699}. ad: CS/Ae. umbellulata.

4.2. Subtilisin inhibitors
 
Si-R1 [{529}].   2R{529}; 2RS{701}. ad: CS/Imperial, Holdfast/King II.
Si-H1 {528}.  [Isa 1 {528}]. 2H {528}. ad: CS/Betzes.
Si-B2 {701}.   1BS {701}. su: Bersee {Koga II}.
Si-D2 {701}.   1DS {701}. v: Koga II.
Si-H2 [{528}], {701}. [Ica 1, Ica 2 {528}]. 1H {528}. ad: CS/Betzes.
Si-R2 [{529}], {701}.   1R {529}, 1RS {701}. ad: CS/Imperial {529}.
tr: Gabo 1BL.1RS {701}.
Si-Sl2 {701}.   1Sl {701}. ad: CS/Ae. longissima.
Si-U2 {701}.   1U {701}. ad: CS/Ae. umbellulata.

Considerable genetic variation for Si-2 was noted in {701}. A location for Si-H2 on 1HL was inferred in {528} but questioned in {701}.

Three subunits of the wheat tetrameric inhibitor of insect "a-amylase, CM1, CM3 and CM16, with homology to the dimeric and monomeric "a-amylase inhibitors and the trypsin inhibitors, were located by Southern analysis of cDNAs pCT1, pCT2, and pCT3 to 4A, 4B, 4D; 7A, 7B, 7D; and 4A, 4B, 4D, respectively {427}.

Genes encoding proteins which inhibit the action of mammalian and insect, but not cereal, "a-amylases were located in chromosomes 3BS, 3DS and 6DS of Chinese Spring {1260}. Also, genes encoding inhibitors of insect "a-amylases were located in H. chilense chromosomes 4Hch and 7Hch {1262}.

4.3. Inhibitors of a-amylase and subtilisin
 
Isa-A1 {908}.   2AL {908}. v: CS.
Isa-A1a {908}.     v: CS.
Isa-A1b {908}.   Null allele. v: Cajeme 71.
Isa-B1 {908}   2BL {908}. v: CS.
Isa-B1a {908}.     v: CS.
Isa-B1b {908}.     v: Bihar.
Isa-D1 {908}.   2DL {908}. v: CS.

Orthologous genes were identified in Ae. speltoides and T. timopheevi {908}. All durum wheats investigated had the genotype Isa-A1b, Isa-B1b.