Laboratory Protocols List

• Company Addresses
  
• Laboratory Safety
  
• Media
  
• Buffers
  
• Phosphate Buffers
  
• Stock Solutions
  
• Antibiotics
  
• Culture Collection
  
• Collecting Cotton Tissue
  
• Tissue Lyophilization
  
• Genomic DNA Purification
  
• Cotton DNA Isolation
  
• Arabidopsis DNA Purification from Nuclei
  
• Plant DNA Extraction Protocol - Meyerowitz Laboratory
  
• Plant DNA Extraction Protocol - PMB
  
• Plant Nuclei DNA Extraction Protocol - DP
  
• Plant Mitochondrial DNA Extraction Protocol - DP
  
• Plant Chloroplast DNA Extraction Protocol - DP
  
• Isolation of Genomic DNA from Azorhizobium caulinodans ORS571
  
• DNA Extraction from ORS571
  
• DNA Extraction Procedure for Rhizobium spp.
  
• General DNA Extraction Procedure for Bacteria
  
• ReadyAmp™ Genomic DNA Purification System
  
• Quiagen Preps
  
• Lessons In Paranoia
  
• RNA Preparation Methods
  
• RNA Extraction from Cotton
  
• Plant RNA from Potato
  
• Isolation Of RNA From Bacteroids and Bacteria
  
• Transformation Competent Cells
  
• Transformation
  
• One-step Transformation of E. coli
  
• Transformation of Yeast - LiAc Method
  
• X-Gal
  
• Large Scale Isolation of Plasmid and Bacterial DNA
  
• Alkaline Lysis DNA Purifications from Bacteria
  
• High-Copy Plasmid Purfication Using The TL-100 Rotor
  
• Xanthomonas campestris Plasmid DNA Mini-Prep
  
• Plasmid Isolation - Medium
  
• Plasmid Isolation - Intermediate
  
• Plasmid Isolation - Large
  
• Modified Eckhardt Gel Procedure
  
• DE52 DEAE Cellulose Phage Lambda DNA Purification Maxi-Prep
  
• Phage Lambda DNA Purification Maxi-Prep
  
• TL-100 Phage Purification
  
• TL-100 Phage Rapid Purification Protocol
  
• P1 DNA preparation
  
• P1 DNA PEG precipitation
  
• Fungal DNA Isolation
  
• Single-Fly DNA Preps for PCR
  
• Cesium Chloride Gradients
  
• Restriction Enzyme Buffer Recipes
  
• Restriction Enzyme Buffer Mixes
  
• Restriction Enzyme Digest
  
• Agarose Gel Electrophoresis
  
• Molecular Weight Standards
  
• Methylene Blue DNA Staining
  
• Glassmilk DNA Purification
  
• DNA from Low Melting Temperature Agarose
  
• Precipitating DNA
  
• Quantifying DNA
  
• Dephosphorylating DNA
  
• PCR
  
• Cloning Blunt-end PCR Fragments
  
• TA Subloning of PCR Fragments
  
• PCR site-directed mutagenesis
  
• Subloning of PCR Fragments
  
• RT-PCR
  
• Differential Display-Reverse Transcription-PCR
  
• Optimized Welsh DDRT-PCR Protocol
  
• DDRT-PCR Eluting Bands
  
• Microplate Templates
  
• PCR Error Rates
  
• Differential cDNA Screening Procedures
  
• Southern Transfers
  
• Southern Transfer - Sponge Method
  
• Hybridizations
  
• Hybridization Conditions - Northerns
  
• Post Hybridization
  
• DNA Probes
  
• Radioactive Labelling of DNA
  
• Randon Primer Oligo-labelling of DNA
  
• Random Primer Method
  
• Spin Columns
  
• PCR Protocol
  
• PCR Amplification from Microbial Colonies
  
• RAPDs
  
• Extra Long PCR (Church Lab)
  
• Sequencing Protocols
  
• Exonuclease Protocols
  
• Misc.
  
• Mutagenesis
  
• Equilibrating Phenol
  
• Siliconizing Glassware
  
• Preparing Salmon Sperm DNA
  
• Cleaning Radiation
  
• Clean-up for Ethidium Bromide
  
• Removal of CsCl/EtBr Waste
  
• Arabidopsis Seed Collection
  
• Arabidopsis Transformation
  
• Arabidopsis Vacuum Infiltration
  
• Preparation of DNA from Single Rice Seedlings
  
• Isolation of RNA and DNA from Rice Seedling Mitochondria
  
• A simple screning method for transgenic rice tissue based on PCR
  
• Non-aqueous Method for Purification of Cytoplasmic DNA from Individual Rice Plants for RFLP Analysis
  
• Enhancement of Division Frequency of Protoplasts and Plant Regeneration in Rice
  
• Isolation of RNA and DNA from different rice tissues
  
• Improved histochemical staining for beta-D-glucuronidase activity in monocotyledonous plants
  
• A rapid, reliable method for preparing somatic chromosomes of rice
  
• cDNA Topics
  
• Misc. Methods (DUMP FILE)
  
• Misc. Stocks
  
• Tissue Culture Stocks
  
• Misc. Strains
  
• A. Thaliana Topics
  
• Preparation Of Nylon Filters For Dot-blot DNA Hybridization
  
• Xanthomonas campestris Tri-Parental Mating Protocol
  
• Agrobacterium-mediated Transformation of Cotton
  
• MOPS Minimal Medium for Xanthomonas campestris
  
• Xanthomodadin Extraction from Xanthomonas campestris
  
• Bacteroid Isolation
  
• Quick Test For Restriction Enzymes
  

  Other Collections

• NWFSC Methods - Mark Strom (ORIGIN)
  
• Protocols for Recombinant DNA - Roe Lab (ORIGIN)
  
• Brendan Babb's BBHelp - Zappe Techniques Course Files (ORIGIN)
  
• Electroporation Methods - Eppendorf
  
• BACs@CalTech - M. Simon (ORIGIN)
  
• BACs@TAMU - B. Creel (ORIGIN)
  
• Bowtell Lab Manual v31.8.95 (ORIGIN and UPDATE)
  
• Biochemical Techniques - Bowtell (ORIGIN and UPDATE)
  
• Cellular Biology Techniques - Bowtell (ORIGIN and UPDATE)
  
• RNA Isolation Methods - Faulkner-Jones (ORIGIN and UPDATE)
  
• RNA Analysis Methods - Faulkner-Jones (ORIGIN and UPDATE)
  
• In-Situ Hybridization to mRNA - Faulkner-Jones (ORIGIN and UPDATE)
  
• Jim Brown's Protocols - Indiana Univ. (ORIGIN)
  
• Promega Protocols (ORIGIN)
  
• University of Minnisota PCR
  
• Chemical Prep - Apx J
  
• Pseudomonas Methods - Goldberg (ORIGIN)
  
• Donnis-Keller Lab Lab Manual (ORIGIN)
  
• FAQlist
  

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