Goldberg Lab Standard Protocols

Table of Contents

  1. Methods and Procedures

    1. Standard (alkaline lysis) mini-prep
    2. GeneClean© II kit
    3. Preparation of electrocompetent cells
    4. Electroporation
    5. ELISA inhibition assay
    6. Immunodot assay
    7. Transformation of competent cells (RbCl2 method)
    8. Partial endonuclease digestion
    9. Wizard© miniprep kit
    10. SDS-PAGE
    11. LPS recovery
    12. Lyophilizing cells
    13. Preparation of phage lysates
    14. Silver staining of PAGE gels
    15. Western blotting (semi-dry)
    16. Tricine SDS-PAGE
    17. Rapid genomic DNA isolation from P. aeruginosa
    18. Altered Sites© mutagenesis protocol

  2. Media, Buffer and Reagent Recipies

    1. Mini-prep solution I
    2. Mini-prep solution II
    3. Mini-prep solution III
    4. 3 M sodium acetate
    5. 10x TE buffer
    6. LB broth
    7. M9 media
    8. SOC broth
    9. 2 M magnesium stock
    10. 1 M glucose
    11. 300 mM sucrose
    12. p-nitrophenyl phosphate disodium substrate buffer
    13. Phospate buffer
    14. Skim milk solutions
    15. 10x PBS
    16. SOB broth
    17. Transformation buffer 1
    18. Transformation buffer 2
    19. 0.5 M MOPS
    20. 1 M potassium acetate
    21. 7.5 M ammonium acetate, pH 7.5
    22. 2 M ammonium acetate, pH 4.6
    23. SDS-PAGE separating gels
    24. SDS-PAGE stacking gel
    25. 30% acrylamide/0.8% bisacrylamide
    26. 4x Tris-Cl/SDS, pH 6.8
    27. 4x Tris-Cl/SDS, pH 8.8
    28. 5x SDS/electrophoresis buffer
    29. 2x PAGE sample buffer
    30. Terrific broth
    31. TNE buffer
    32. lambda-Medium
    33. lambda-Top agar
    34. lambda-Diluent
    35. 1 M magnesium sulfate
    36. Silver stain fixative I
    37. Silver stain fixative II
    38. Silver stain stop solution
    39. Silver stain kit reagents
    40. Western blot transfer buffer
    41. TSDS-PAGE separating gel monomer
    42. TSDS-PAGE stacking gel monomer
    43. TSDS-PAGE anode buffer
    44. TSDS-PAGE cathode buffer
    45. TSDS-PAGE gel buffer
    46. TSDS-PAGE stacking gel
    47. TSDS-PAGE separating gel

  3. Figures and Tables

    1. ELISA inhibition plate
    2. Partial endonuclease digestion diagram
    3. Separating gel table
    4. Calculating phage titers
    5. Phage media diagram
    6. Silver stain protocol
    7. Western blot transfer stack assembly
    8. Western blot transfer times
    9. DNA digestion using rapid P. aeruginosa genomic prep

Back to the Channing Home Page. Back to the Protocols Page.
Web page maintained by: Michael Coyne.
Revised: Sunday, September 03, 1995 1:24:52 PM