Collection and Storage of Cotton Leaf Tissue

1. Harvest cotton tissue of interest. Separate the different leaf sample in a plastic bag.

2. Chill the samples on ice for transport back to the laboratory.

3. Remove the midribs, if desired, then put the each sample in a 50 ml polypropylene tube (PPT).

4. Place the samples into -70 C until freeze-drying them.

5. Freeze-dry (See Lyophilization) the sample for 2 to 4 days.

6. Store the freeze-dried leaf tissue in a 50 ml PPT tightly-capped at -20 C or in a desiccator at room temperature.