Phosphate (Sodium) buffer Chart

2 M monobasic sodium phosphate, monohydrate (276g/L)

Stock solution B

2 M dibasic sodium phosphate (284 g/L).

Mixing an appropriate volume (ml) of A and B as shown in the table below and diluting to a total volume of 200 ml, a 1 M phosphate buffer of the required pH at room temperature.

 A	B	pH

 92.0	 8.0	0.8

 90.0	10.0	5.9

 87.7	12.3	6.0

 85.5	15.0	6.1

 81.5	19.5	6.2

 77.5	22.5	6.3

 73.5	26.5	6.4

 68.5	31.5	6.5

 62.5	37.5	6.6

 56.5	43.5	6.7

 51.0	49.0	6.8

 45.0	55.0	6.9

 39.0	61.0	7.0

 33.0	67.0	7.1

 28.0	72.0	7.2

 23.0	77.0	7.3 

 19.0	81.0	7.4 

 16.0	84.0	7.5 

 13.0	87.0	7.6

 10.5	89.5	7.7

 8.5	91.5	7.8