Enzyme	REact	Unit/µl	Incubation temp

Acc I	1	5
Apa I	4	20
Ava I	2	10
Ban I	5
Bam HI	3
Bcl I	2	10	55 C
Bgl I	2	10
Bgl II	3	8
BstN I		10	60 C
Cfo I	1
Dra I	1	40
Eco RI	3	20
Eco RV	2	10
Hae III	2	10
HindIII	2
Hinf I	2	10
Hpa II		10
Kpn I	4	10
Mbo I	2	10
Mlu I	3	8
Nae I	4
Nde I		40
Nhe I	4	10
Not I		10
Nru I	7	10
Nsi I	5
Pst I	2	10
Pvu I		10
Sal I		20
Sca I		10
Sfi I		10
Sma I	4	6	25 C
Ssp I	6
Sst I	2	5
Taq I	2	14	65 C
Xba I	2	20
Xho I	2	15