Details of 78 EST-derived SSRs, their primer sequences and the expected product size

Source sequence	Primer name	Forward Primer	Reverse Primer	Product size	Tm	Functional?	EST's best BLAST hit
TaLr1130B07A	PK 1	GCCTTCGCCACCAACTTC	CACGACGTACAAGTACACATGC	101	62	Yes
TaLr1130B01A	PK 2	GATCGAAGGCGTCGCTCT	CTCCACGTACGTCACGCC	98	59	Yes
TaLr1130H05A	PK 3	TTGCTGAGGTGGTGTTTCCT	TAAGGGCTCCTCAAGCTCCT	101	61	Yes
TaLr1130B02A	PK 4	GGTATTGTGGCTTTCGCATT	TACCATTACCGGTTGCGACT	98	59	Yes
BI480021	PK 5	GAGGCGGCCTACAAGAAGAT	GAGGCGGCCTACAAGAAGAT	203	60.73	Yes
BG909793	PK 6	GCTAGGGTTTCAGGCTCCTC	GCTGGCATCTAGGTGCGCCC	196	69.51	Yes
BG909784	PK 7	CACCGACGAGGAGAGGATAA	GGCGCTTGTTGATGTCCT	188	60.21	Yes	fasciclin-like arabinogalactan-protein 1(Arabidopsis thaliana)
BG909764	PK 8	GCTAGCAACCAGAGACTCAC	GTCGTCCTCGTCGTTGTTG	246	60.3	Yes
BG909482	PK 9	GTCCAGCTCTCGGTACTTGG	TGCATCCAAACAAGCCATGC	201	65.34	Yes
BG909325	PK 10	CAAGCCCATCCCCATCGCCG	AAAATTCCACCTGCTTGACG	199	73.93	Yes	unknown protein(Arabidopsis thaliana)
BG909030	PK 11	GCCACTCACTCATCGCTACA	CTTAAAAAGGCCGAGCAGTG	208	60.02	Yes	ferredoxin-NADP(H) oxidoreductase (Triticum aestivum)
BG908985	PK 12	TTTCTCAGCTTTTCTTCTCC	GGCGTCCTTCCCCTTCTT	100	61.51	Yes
BG909152	PK 13	GCCTACGCAGACCGACTTCC	TGAGGATGTACGGGGAGAAG	209	64.42	Yes	AT5g40270/MSN9_170
BG908960	PK 14	AGCTTGGCCCTCTTCTCGCC	CAGAGGCCAGTGCATTTCTC	193	67.4	Yes	thylakoid lumenal 29.8 kDa protein chloroplast precursor (Mouse ear cress)
BG908922	PK 15	GACGACGACGAGGACGAG	GACGTCGGTGGTGTAGGTCT	134	60.58	Yes
BG908890	PK 16	CAGCGTAGGGATCTGAATGG	CTGCCAGATACAACGGCGTC	177	64.01	Yes
BG908909	PK 17	GTGGAGAAGCCGAAGATGAC	GTAAGCAAGCGCTCCAGAAT	200	59.81	Yes
BG908875	PK 18	AGACGGCGCGCATGGCGA	AAGTCTACGCGGGTGAGGT	217	68.85	Yes	NIFS-like protein CpNifsp precursor(Arabidopsis thaliana)
BG908846	PK 19	CGCCAGAGCAATTACTGACC	GGGGAAAATATATGCGGAAAA	203	60.8	Yes	polyubiquitin 4 (wild oat)
BG908818	PK 20	CGACGATTCCAATCACGAG	CCCATGGGTGTTGACTCTG	195	60.21	Yes
BG908816	PK 21	CTAAGCCTAAGGCGGGGGAG	TCCCCTCCTCGATGTCTTC	195	64.35	Yes
BG908811	PK 22	CACCACGTTCCACGCTACTA	GCTGCTGCTCTTCGTGGT	202	60.3	Yes
BG908791	PK 23	GCATGAATTGTGCATATCGAG	TCCAACCATTCCAATCAACA	198	59.75	Yes	fructose-bisphosphate aldolase chloroplast precursor (EC 4.1.2.13) (ALDP) (Rice)
BG908790	PK 24	GTGGCAGGCAGGCAAGCAAG	TGACGAGCTCATCGTCGTAG	177	68.7	Yes
BG908612	PK 25	AAGAATAGGAGTTTAGGACC	CCTGAGGCGATACAGGATGT	166	60.1	Yes	unknown protein (Arabidopsis thaliana)
BG908603	PK 26	GCAACCGTGCGCGCGACTCC	CGGCACCTCGTAGTACTGG	216	75	Yes
BG908569	PK 27	TGTACTACGCCTGCATGCTC	GTGCTCAAGGACATCGGGTA	206	61.07	Yes	putative serine-glyoxylate aminotransferase (Fritillaria agrestis)
BG909413	PK 28	CGATCCATGCCATATCACAG	CCGCTTGTAACTTGGACACA	181	59.91	Yes	probable (S)-2-hydroxy-acid oxidase peroxisomal 2 (EC 1.1.3.15) (Glycolate oxidase 2) (GOX 2)
BG909459	PK 29	TACTAGAGGAGATGCAGACG	TGGCAATAGCCTGAACCACT	210	60.66	Yes	unknown protein (Arabidopsis thaliana)
BI479713	PK 30	TTCCACCCCAGACTCGCG	GTACGGGCCAACCTTGTTTA	177	52.45	No	endomembrane protein EMP70 precursor isolog (Arabidopsis thaliana)
BI479118	PK 31	GATGGCTCCCGTAAAGATCA	TTTCCATTTTCGGAAAGCAC	212	48.35	Yes
BI479123	PK 32	GCGAGGCTCAACCAAGAG	GCAAGGCTGGCTGAATCTAC	187	52.35	Yes	rab28 protein (maize)
BI479585	PK 33	ATGACCGAGGTCGTTCAGAT	CCTTTGCATTGCTACCGTTC	199	50.4	No
BI479751	PK 34	CGCAAATCGCAACCTTATTT	CCTTGGAGATCCACAGATCC	208	49.4	Yes	probable phosphoprotein phosphatase (EC 3.1.3.16) (apple tree)
BI479875	PK 35	TCCCCAAAGATCCAGATCAC	CGTCACCACGATCTTGTTCC	201	51.45	Yes	chitinase-like protein 1 (Arabidopsis thaliana)
BI479884	PK 36	AATCGAGTGCACTCGTCG	GGGTTGAGACCAAGTTCACG	204	51.2	No
BI479902	PK 37	TCCGACGACCACCACCGAC	ACATGTACCTCGCGCCATC	187	54.45	Yes	putative NADH-ubiquinone oxireductase(Arabidopsis thaliana)
BI480011	PK 38	AGGATGACGACCAGGACAAC	GCTCCTACAAGCCCACACAT	208	52.5	Yes
BI480021	PK 39	GAGGCGGCCTACAAGAAGAT	ATGCTTATTCTCGCAACACA	203	49.4	Yes
BI480308	PK 40	ACCCTGCTCCTCAACCCCCTT	CAGCTTCTTCAGCTGTGTGG	203	54.5	No	ubiquitin-like protein SMT3(Rice)
BI479142	PK 41	GAAGGCAGAGGAAGGGACAT	CTTGTTCAGCCTCACCACCT	225	52.5	No
BI479580	PK 42	CGAGCACGAGAAGCTAAACC	GGTGTCACCCGCACTAGG	197	60.15	Yes
BI479694	PK 43	GTTCTCTTCGCGGCTTTTC	ACAGCCTGTCCGAGATGAAG	205	51.4	Yes
BI479738	PK 44	CCTCCGATCCAAACCCTAG	ATGTAGTTGCCGGTGTAGGC	224	52.5	No
BI479767	PK 45	GCTCAAGCACAACGAAATCA	CCTTCCCAACTGAAACGAAT	204	48.4	Yes
BI479770	PK 46	TCTCAAGAAACCCCTGCTTG	CAGGGCCTTCTTCTCCTTG	196	51.4	No
BI479809	PK 47	TGCTGTTCGACATCATCCTC	CCAGGCATTTTCCTTTCTGA	182	49.4	Yes
BI480549	PK 48	GACCAGATGAAACCGCTGAT	AACCCCTTGTTGCACACCT	242	50.25	Yes
BG604521	PK 49	CGACGCCATTCCTGAGAATA	CTTCCCTTGCTTGCTGACAT	196	50.25	Yes	protein F23N19.9 [imported] (Arabidopsis thaliana)
BG605288	PK 50	GTGGTGGTGGAACCTCCCG	GACCTGCGCCATTGTGTC	229	54.3	No
BG605281	PK 51	TACCCGAACCTTCTCGACTC	CCGAAGTGGAAGAGGTCGTA	226	52.5	Yes	probable choline-phosphate cytidylyltransferase (EC 2.7.7.15) (clone CCT1) (rape)
BG605310	PK 52	TTCTTCCCTCCGAATTTCCT	CAGCATTGGCGACAGACC	201	61.45	Yes
BG606122	PK 53	GATTTGGGGCCTAGGGTTAG	CATGTCATCCCTCTCCTCGT	197	60.07	Yes	hypothetical protein F23H24.4 [imported] (Arabidopsis thaliana)
BG604565	PK 54	GTGTCATCCCTCTGGTCGAT	ATATGCATGCCTTGTTGTTG	191	59.93	Yes	homologue to gamma-gliadin (Triticum aestivum)
BG605476	PK 55	TCTCCCTCCTCAGCTACGAC	AGCACGGGCGTCGACATGGC	133	73.16	Yes
BG604672	PK 56	CTCGCTCCTCGCCGTCGTCC	TGGAAGTAGTGAGCCGTGAA	228	72.03	No	At2g24280/F27D4.19 (Arabidopsis thaliana)
BG604688	PK 57	AATTGGAAAAGGTGGTGACG	GTAACTACCCCTGGGGAAAA	201	59.83	Yes
BG604694	PK 58	GTCCTCGTGCTAGGGACCTT	GTGAGCACACAGCAGCAGTT	197	60.65	Yes
BG604714	PK 59	AGCGTCGCGAGGGAGACA	TGAAGTTCTCAGGATCCAAGC	188	65.73	No	cytochrome b5 reductase (Zea mays)
BG604731	PK 60	AACCCTGATTGAAACGACC	CCTCCAAGCCGGCGGGCAA	102	74.77	No
BG604862	PK 61	CTTGCCCCCTCTCGCCCC	TCCGGACGACATTCTCTCTC	169	69.71	Yes	hypothetical protein M3E9.90 (Arabidopsis thaliana)
BG604939	PK 62	GCCGCCCACTCCGACCTCGC	TGGTTAGGATGTTGCTGCAC	213	75.61	Yes	probable splicing factor [imported] (Arabidopsis thaliana)
BG604973	PK 63	GATGAGCACACGATCGAGAC	ATGCACCTTCCTTTTGCTCA	199	60.78	No
BG605043	PK 64	CGATCCCTCTGCTGCATT	AGAGCTCTCGCTTCCTCGTA	196	59.9	Yes
BG605005	PK 65	CTCTTCCTCGCAGCAGCA	CGGCGTGCTTGTAGTGGTAG	220	61.79	Yes	pit2 protein (clone pAL141) Pi starvation induced (common tobacco)
BG313284	PK 66	CTACAAGATCGGGGAGGACA	CTAGTACCTCCCGCACATGG	203	60.53	Yes	phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (PEP carboxykinase)
BG313511	PK 67	TCCTTGGTCACTCTCCAAACTT	GAACGTGAGAAGATATAT	209	60.15	Yes
BG313574	PK 68	TCTCCTTCCCCCACGGTGC	TGGGCGATGTAGTACGTGTC	182	68.01	No	hypothetical protein [imported] (Arabidopsis thaliana)
BG604260	PK 69	CTCTCCCGCCTCTCCAAG	CTCGTCCGCCTCCTTCAC	150	61.97	Yes
BG604521	PK 70	CGACGCCATTCCTGAGAATA	CTTCCCTTGCTTGCTGACAT	196	61.13	No	protein F23N19.9 [imported] (Arabidopsis thaliana)
BG262504	PK 71	CGGGAGCGGTCGAGCGGCGC	GCTCGTAGGCTTTCTTGAGC	198	79,93	Yes	putative MADS-domain transcription factor (Zea mays)
BG262523	PK 72	GAGGCTAGGGTTTCCTCCAA	AGCCCGACGAAGTCAAAGT	224	60.57	Yes
BG262529	PK 73	GAGCTTCAGGGTCAAATGGA	TCCTCGTCCTCATCATCCTC	197	60.2	Yes	nucleosome assembly protein I
BG262603	PK 74	ATCCGTGCCATCGTCTACTC	GTTGTTGTTGAGGCTGCTGA	199	60.1	Yes	low-molecular-weight glutenin subunit group 5 type III
BG262485	PK 75	GTTCACGCAGCAGCCCACCAG	CCTCTCCTCCTCCTCGTTCT	211	70.7	Yes	hypothetical protein F25E4.180 (Arabidopsis thaliana)
BG274944	PK 76	GCGCATACGATACGAGCGAG	GCCTCCATTTCTGAGGATGA	209	64.13	Yes	unknown protein (Arabidopsis thaliana)
BI479561	PK 77	GCAGTCGAACCTGAGACCAT	ATCGTAGAGCTGGAGGACGA	177	60.27	Yes
BG263001	PK 78	ACGCCAAGATCATCAACGAC	GCCGCGATGGCCGCAGTA	203	61.08	Yes