Development of
microsatellite markers for wheat genetic mapping improvement: outcomes from the
wheat Genoplante program.
Pierre SOURDILLE1,
*, Béatrice GANDON1, 2, Nathalie NICOT1, Vincent
CHIQUET1, 2, Liyi Zhang1,
Sébastien SPECEL2, Nathalie FOISSET2, Philippe DUFOUR2,
Alain MURIGNEUX2 and Michel BERNARD1
Addresses :
1 UMR INRA-UBP Amélioration et Santé des Plantes, Domaine de Crouelle, 234 Avenue du Brézet,
63100 CLERMONT FERRAND, FRANCE
2 BIOGEMMA, Campus Universitaire des Cézeaux,
24, Avenue des Landais, 63170 AUBIERE, FRANCE
* Corresponding
author: (33) 4 73 62 44 37, pierre.sourdille@clermont.inra.fr
ABSTRACT
Microsatellites have shown their
great efficiency for wheat genetic mapping and for establishment of saturated
linkage maps on every agronomical cross of interest. In this project, six microsatellite enriched libraries were produced using as
DNA source the wheat diploid ancestors T.
urartu, Ae. speltoides and T. tauschii (two
libraries) or polyploid wheats
like T. durum cv
Langdon and T. aestivum
cv Renan. More than ten thousand of genomic clones were sequenced on both sides
(21,037 sequences) and analyzed for redundancy leading to 5,593 contigs. Primer pairs could have been design for 40% of
them. More than 80% of the primer pairs gave an amplification product and about
60% of them were polymorphic on a set of eight wheat lines and mapped (1,101
loci). Simultaneously, genomic low copy and EST sequences were screened for the
presence of a microsatellite motifs.
About 10% of the EST contigs contained a SSR and a
set of 1,497 primer pairs was designed and evaluated for polymorphism on the
same eight varieties. The level of polymorphism was lower compare to the
genomic SSRs (40%) but the profiles were of better
quality. Overall, about 1,700 new loci were mapped during this program.
Together with the public SSRs, the coverage is now of
about 1 SSR each