All the processed ESTs have been submitted to GenBank at NCBI. The information on
genbank accession number and dbEST number for all the ESTs can be accessed from GenBank Number site. The GenBank numbers are
organized by libraries. The EST name used
for GenBank submission has been modified slightly from the EST name used in the fasta file. The EST name in the fasta file
represents (plate number)_(96-well plate position)_(384-well plate position)Z_(numbers and letters generated from
sequencing machine). The EST name used for GenBank submission is WHE(plate number)_(96-well plate
position)_(384-well plate position)Z(a designated letter for sequencing primer used).
Example: Albany EST name - 2221_A04_A07ZS5_022.ab1, GenBank EST name - WHE2221_A04_A07ZS.
Example: Albany EST name - 2257_A01_B01Z_002.ab1, GenBank EST name - WHE2257_A01_B01ZE.
Sequencing primers and their designated letters:
- BKrev - V
- M13 forward - M
- M13 reversed - R
- Oligo dT - O
- Stratagene's pBSK reversed - B
- Stratagene's SK - S
- Stragagene's KS - K
- Clontech's MMAD3' - A
- T3 - E
- T7 - T
- Oligo dT wobble primer (an equal mixture of (T)27A, (T)27G and