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GrainGenes Environment Report: Fargo, ND Greenhouse

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Environment
Fargo, ND Greenhouse
Location
Fargo, ND
Remarks
Two independent greenhouse experiments were laid out in a randomized complete block design (RCBD) with three replicates in each experiment. Barley genotypes were grown in the greenhouse at Fargo, ND and temperatures were maintained at 22 to 25 C with a photoperiod of 14 h light and 10 h dark with supplemental lighting provided by 1,000 W, metal halide bulbs. Five seeds of each line were sown in a Ray Leach 'Cone-tainer' 3.8 cm diameter x 14 cm depth (Stuewe & Sons Inc., Oregon) (the experimental unit) filled with Sunshine mix no. 1 (Sun Gro Horticulture, Agawam, Massachusetts) and plants were fertilized (3 g liter-of 15-30-15 Miracle Gro, Scotts, Port Washington, NY) as needed. The two to three leaf stage of barley seedlings were inoculated with a suspension of spores from 12- to 14-day-old cultures (5,000 conidia ml−1 suspension) using an atomizer (DeVilbiss Health Care, Inc., Somerset, PA) pressured at 25 to 30 kPa. Spray inoculation was continued until run off. The seedlings were dried at room temperature for 20 to 30 min and then incubated in chambers humidified with ultrasonic misters (HM-470, HOLMES Product Corporation, Milford, MA), 10 s continuous misting at 4 min intervals for 24 h in the dark at 21C. After 24 h incubation in the misting chamber, the plants were slowly dried at room temperature.
Trait Study
TCAP Barley SFNB Greenhouse Evaluation
QTL
QSfnb-2H-8-10
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