Query (optional)   in Class  

GrainGenes Gene Class Report: Nucleolus organizer regions

[ Printable Version ]  [ Submit comment/correction ]

Gene Class
Nucleolus organizer regions
Reference
ReferenceMcIntosh RA et al. (1995) Catalogue of gene symbols for wheat. Proceedings of the 8th International Wheat Genetics Symposium 1333-1500.
[ Show all 11 ]
Abbreviation
Nor
Character affected
Ribosomal RNA
Orthologous Gene Set
Nor-1
Nor-2
Nor-3
Nor-4
Nor-5
Gene
Nor-R1 (Secale)
[ Show all 24 ]
Locus
Nor-R1
[ Show all 12 ]
Clone
pTa250.15
Comment
18S-5.8S-26S rRNA genes.
NORs have been observed as secondary constrictions associated with nucleoli on satellited chromosomes (e.g., 167), and by in situ hybridization to chromosome spreads (34, 207, 721) of 18.S-5.8S-26S ribosomal-DNA probes (32, 301). Allelic variation in gene number has been demonstrated at all wheat Nor sites and at Nor-R1 by filter (252) and in situ hybridization (720).
Allelic variants of the Nor loci are detected by hybridization of rDNA probes to restriction endonuclease-treated DNA on Southern blots (33, 203, 657, 1005). Alleles Nor-B2a to Nor-B2f were identified using Taq1 digests of genomic DNAs hybridized to derivatives of the plasmid pTa250 (301) containing spacer-DNA fragments pTa250.4 (252, 657) and pTa250.15 (203).
Other variants may have been isolated (1005) using BamH1/EcoR1 double digests and pTa71 (301). The variants Nor1a and Nor2a in Maris Huntsman, Nor1b and Nor2b in Bezostaya 1, and Nor1c and Nor2c in Maris Ranger and Cappelle Desprez (1005) may or may not be equivalent to the Nor-B1 and Nor-B2 alleles listed in the Catalogue of Gene Symbols for Wheat (9441).
Wheat Gene Catalog Reference
9441
ReferenceMcIntosh RA et al. (1995) Catalogue of gene symbols for wheat. Proceedings of the 8th International Wheat Genetics Symposium 1333-1500.
[ Show all 11 ]