This is the first described advanced-backcross QTL using spring barley as a model, and is the first barley QTL study based exclusively on SSR markers. The mapping population was created from a cross between Hordeum vulgare (Apex) and Hordeum spontaneum (ISR-101-23) followed by 2 cycles of backcrossing with Apex and one cycle of selfing. 136 randomly chosen individuals were used for genotyping and these were subsequently bulk propagated for field testing; BC2F2:5 in 1999, and BC2F2:6 in 2000.
13 quantitative traits were measured in up to 6 environments (3 sites over 2 years), resulting in 86 putative QTLs among 585 marker x trait combinations.
SSR markers polymorphic between the parents but failing to show the ISR101-23 allele in the BC2F2 population are assigned an 'Error 1' to their map position resulting in placement on the map in a column to the right of markers with both alleles found in the BC2F2 population. QTLs are in the right-most column on the interactive maps.