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GrainGenes Map Data Report: Rye, ExR

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Map Data
Rye, ExR
Map
Secale-ExR-1
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Species
Secale cereale
Female Parent
E-line (rye)
Male Parent
R-line (rye)
Type
Genetic
Map Units
cM (Kosambi)
Reference
ReferenceLoarce Y et al. (1996) A molecular linkage map of rye Theoretical and Applied Genetics 93:1112-1118.
Contact
Loarce, Yolanda
Remarks
DNA extracted from young leaves of parental lines and 54 F2 plants was digested with DraI, EcoRI, EcoRV, HindIII, XbaI, or XhoI, electrophoresed, and Southern blotted. Probes were labelled with Digoxigenin-11dUTP by PCR prior to probing, and RFLPs were detected by enzymatic reaction to anti-Digoxigenin-AP and Lumigen-PPD. Of 180 arbitrary-sequence oligonucleotides (10-mers, Operon Technology) used as single primers, 162 produced scorable fragment patterns; however, only 5.5% of bands were polymorphic. Mapmaker V.3 software was used for linkage analysis of 87 RFLPs and 17 RAPDs. Chromosomal affiliation of each linkage group was established by hybridization of selected probes to wheat-rye addition lines. Arm assignments were based on previously-known positions of 2 or more markers.
DATA TYPE F2 INTERCROSS
90 54 0
Image
ExR 1R map
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Locus
MWG573DBAC-HA-DHBHHHHAAHABHA-ABBH-HHHHHHAABB--HHHH-BABH- --C-
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