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GrainGenes Map Data Report: Rye, ExR

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Map Data
Rye, ExR
Map
Secale-ExR-1
[ Show all 6 ]
Species
Secale cereale
Female Parent
E-line (rye)
Male Parent
R-line (rye)
Type
Genetic
Map Units
cM (Kosambi)
Reference
ReferenceLoarce Y et al. (1996) A molecular linkage map of rye Theoretical and Applied Genetics 93:1112-1118.
Contact
Loarce, Yolanda
Remarks
DNA extracted from young leaves of parental lines and 54 F2 plants was digested with DraI, EcoRI, EcoRV, HindIII, XbaI, or XhoI, electrophoresed, and Southern blotted. Probes were labelled with Digoxigenin-11dUTP by PCR prior to probing, and RFLPs were detected by enzymatic reaction to anti-Digoxigenin-AP and Lumigen-PPD. Of 180 arbitrary-sequence oligonucleotides (10-mers, Operon Technology) used as single primers, 162 produced scorable fragment patterns; however, only 5.5% of bands were polymorphic. Mapmaker V.3 software was used for linkage analysis of 87 RFLPs and 17 RAPDs. Chromosomal affiliation of each linkage group was established by hybridization of selected probes to wheat-rye addition lines. Arm assignments were based on previously-known positions of 2 or more markers.
DATA TYPE F2 INTERCROSS
90 54 0
Image
ExR 1R map
[ Show all 6 ]
Locus
[ Hide all but 1 of 91 ]
MWG573DBAC-HA-DHBHHHHAAHABHA-ABBH-HHHHHHAABB--HHHH-BABH- --C-
OpE5AACCAACCCACCAA-CCCCACCCCCA-CCCCCACCCCCCCCCCCC-CACC AA--
OpG4AACCCCCCCCCCACCACCC-CCCCCACCCC-CC-CCCCCACCC-CCCCAC CAAC
OpJ5ADDBDB-D-BDDBDDDD---DDBDDBDD-BD-DD-DDDBDDDBDDDB-DDD ----
OpJ5BDDBDB-D-BDDBDDDDDD-DDBDDBDD-BD-DDDDDDBDDDBDDDB-DDD ----
OpO18-DDBDDBDDDBDDDBDBDBDDDDBDBDDBB-BD-DDBDBDDDDDDBBBD- ----
OpR10DDBDDBDDDDD-DDDDDDDDDDBDDDDDBDDDDBDBDDBDDBDDDBBDDB DDDB
OpR4R11DBDBBDDDDDDBDDDDDDD-BDDDDBBDD-DDDDBDBBDDDDDDDDBBBD DDDB
OpS10-DDBDDBDDBBDDD-DBDDDDD-DDBBDBBDBDDBDBDDDDDDDDD-DDB DDDB
OpS19BACACCAACCCCACCCACCACCCCCCC-CCCCCCCAACCAACCCCCC-CCC AACC
OpS2DDDBDDDDDDD-DDDDBDDBDDDBDDDDDDDBBDD-BDDDD-D-DD-DD- -DDD
OpT18DDDBDDDDDDBDB--DBDDDD--DDB-DBBDBDDBDD-DDDDDDDBDDDB DDDB
OpT6CCCCCACCACACCCCACCCCCCCCACC-CCCCC-ACCACCCCCCCCCCCA CACA
Xbcd1CCCCCCCCACBABBCCCCAAAAACBACCBCCCBCACBCCACACCCBBCCC BAAA
Xbcd1130HBHHAHHBBBHBHBHHHBBAAHBBHHHHHBHCHHHAHAHHHBHHHHCACD CDA-
Xbcd115CAACCCCCC-CCAAC--CCCCCACCCCCCCCCCCCCACCAC-CCCAACCA CA--
Xbcd1434HHHHHHBHBABBAAAAHHABHHAHHHBHHAHHHHBBABAHHHHHHAHHHB BAAB
Xbcd147CCCCCCCCACBABBCCCCAAAAACBACCBCCCBCACBCCACACCCBBCCC BAAA
Xbcd221AHHAHBAHAHHHHHBAHHHHBHBBHHHHBHAHHHBHHHHAHHHHHHHBAB AAAH
Xbcd276HHHHHBHHABBABBABHHAAAAAHBAHHBDHHBHAHBHHAHAHHHBBHHH BAAA
Xbcd298ABHHHBAAHAHHABAHADBCAHAHHHHHHBDHBAHBHHHHDHHHHHBHCHH CH--
Xbcd298BAHHAABAHAHHHHABAHHHHBHBBBBBHBDHHHHBBHAHAHHHHHHHHAH HH--
Xbcd327ABHB-AAHHHBHBDHHHCDB--HHBBH-HHCBHDAAHB-DHHHH-BDBH- A-BB
Xbcd351HBHHAHHBHBHHHBHHHBBAAHBBHHHHHBHHHHHAHAHHHHHHHHBABH HAAB
Xbcd385ABABBAAHHHBHBHHAHHABHBHHBBHHHHHBHHAAHBAAHHHHHBABHH AABB
Xbcd402AHHBBBAHHAABACACACHBAAAHHCCHHCCCCCCBCCCCCACAAACHBCH HACC
Xbcd402BHBHBBAHHAABACACACHHAAABHCCHHCCCC-CBCCCCCHCHHHCHBCH HACA
Xbcd451D-D--DD-D-DDDBB-DD--DD--DDD-BBDDDDDDDDD-DD-D-D-DD- ----
Xbcd508HHHHAHHBAAHHHBHHHBBAHHBBHHAHHBHHHHHAAHHHHHHHHHBHHH HAA-
Xbcd758HHHCHHHHA-AAHHHCCCABAHABAHHHHCCCCCAHCACCCCCCCCC-CA CAC-
Xbcd808AHHAABAHAHHHHHBAHHHHBHBBHHHHBHAHHHBHHHHAHHHHHHHBAB ADAD
Xbcd9AHCHAHHBAAHHHBBHHBBHHHBBHHAHBHHHHHHHAHHDHHHHHABHHB HHA-
Xbcd927HHHHHHBHBABBAAD-HHABHHAHHHBHHDHHHHBBABAHHHHHHAHHHB BAAB
Xbcd93ABHB-ABAHHABAHHHAHAHBHHABBH-HBAHAHHABB-HHHHH-HBBA- H-BH
Xcdo1417AH-H--HH-A-HHHBB-DC--HH--HHA-BBHHHHHHAHH-HH-H-A-HH- ----
Xcdo348B-H--AA-A-HABAB-HH--HA--HAH-BBHHAHBHHBH-HH---HHHA- ----
Xcdo358 -CCCCACACCACACCCC--CCCCCCCACCC-CCCCACCCCCC--CCCCA CC-C-
Xcdo395-AHBAABAAHHAHABHBBHAHA-BHHAHBHBBHHHHBHBHHHHHHBBBAB AAH-
Xcdo419AC----AA-A-CACAC-CC--C---CCC-CCCCACCCCCC----C-C-CC- ----
Xcdo419BD-D--DD-B-DBDBD-DB--DD--DDD-DBDBDDDDDDD-D--D-D-DB- ----
Xcdo419CC-C--CC-C-CCCCC-CC--AC--CCC-CCCCCCCACAC-CC-C-C-AC- ----
Xcdo460DA-BAADD---AHAB-HBDAHAHHHHAHBHHCCHHHHHCCHHCHHBCHHC A-H-
Xcdo687--D--HH-B-HBHBH-HB--AH--HHH-HB-CHHHAHAH-AB---HBAB- ----
Xcdo749D-DD--D-B-DBDBD-DD--DD--DDD-DBDBBDDDDDD-DB-D-B-DB- ----
Xcdo772H-D---H-A-ADHHH-HC--HH--AHH-HHHBBCAHHAH-HH---DCAH- ----
Xcdo780D-H--AH-A-AHHHH-HH--HH--AHH-HHHBBHAHHAH-HH---HBAH- ----
Xcdo783A-B--AH-H-HHAAH-HH--HH--HAH-BBHHAHBBHHB-HB-H-B-BA- ----
Xcdo786AHBHH-HH-BBHBHBHHHBBAAHBBHHH-HBHBHHHAHA--HHHH-HBAB- --A-
Xcdo786BAACC-AC-CA-CAACCCCC-CCCCCAC-CCACAACCCC---C-C--CAC- ----
Xcdo786CAA---A--CCCCAA-A-CCACCCCCA--CCACA-------------CAC- --C-
Xcdo786DAAC--AC-CACCAACCCCCACCCCCAC-CCCAACCCCC--CCCC--C-C- --C-
Xcdo836HHHH-HH-ABB-BBHCHHAAAA-HBHH-CHHHCHAHBB--HA-H-BBHH- --H-
Xcdo942ACCC-CC-CCACACCCACACCC--CCC-CCACACCACC--CCCC-CACA- --C-
Xpsr103--D--DD-HHCHBHHAAHABHBHHBB--HHHBHHAABB--HHHH--ACH- --C-
Xpsr1077H-H--AH-A-BABDC-BH--AA--HBH-BBCBAHBHHBH-HH-H-B-BA- ----
Xpsr129AAB--AH-HAHHAAHHHHBAHH-BHAH-BHHHAHBBHH--HBHH-BBHH- --C-
Xpsr142HHH--DH-AHAAHHHHBHABAHABAHH-HHHCBHAHBA--HHHH--BAH- --H-
Xpsr156AABDHABACBDDCAB-HHHAAAHHHBH-HHDDAHBHBA--HHHHHD-AA- --H-
Xpsr371HHH--AH-AHHHHHHHHHABHHABAHH-HHHBBHAHHA--HHHH-HBAH- --H-
Xpsr578B----DD-D-DDBDD-B---DD--DDD-DBDBDDBDDBD-DD---BDBD- ----
Xpsr601H-D--HB-B-BBAAH-AD--HH--HHB-HAHHAHBBHBH-HH---AHBH- ----
Xpsr628CCCB-HH-BBHBHBHHHHBAAHBBHHH-HBHCHHHAHA--HBHH-DBAB- --A-
Xpsr637H-H--HH-A-HHHBB-HC--HH--HHA-BBHHHHHHAHH-HH---ABHH- ----
Xpsr910CDCC-ABAHHBAHABABHHAAABBHBH-BBHBAHBHBH-HHHHH-BHBA- A-HB
Xpsr915DDD--DD-DDDDDDDD--DBDDDBDDD-DDDDDDDDBD--DDDD---DD- --D-
Xuah103A--C--AC-A-ACCCC-CC--CC---CC-CCCCCCACCAC-CC---CCCC- ----
Xuah103BD-D--DD-D-DBDDD-DD--BD---BB-DDDDDDBDBBD-DD---DDBD- ----
Xuah103CD-B--BD-B-DBDDD-D---DB---DD-BDDDDDDDDDB-DB---BBBD- ----
Xuah105H-HH--H-A-HHHBB-HA--HH--HHA-BBHHHHHHAHH-BH---HBHH- ----
Xuah30DDDDDDBDBDBBDDDDDDDBDDDDDDBDDDDDDDBBDBDDDDDDDDDDDB BDDD
Xuah39AAABBHHHBHBBAHBHHAHAHHAHHHHHHHHBHHBBAHHAHAHHHHHBHA BADH
Xuah48A-C--CC-C-CCACC-CC--CC--CAC-CCCCCCCCCCC-CC---CCCC- ----
Xuah5ABABBHAHHHABHHHHAHAH-HHABBBHHHHHHHAHBBHHHHHHABABAH HABB
Xuah52ABABBHAHHHHHHHHAAHABHBHABBBHHHHHHHAABBHHHHHHHBABHH AABB
Xuah58AD-D--DD-B-DBDBD-DB--DD--DDD---D-DDDDDDD-DD---DDD-- ----
Xuah58BC-C--CC-C-CCCCC-CC--AC--CCC-CCCCCCC-CAC-CC---CCAC- ----
Xuah6AABABBHHBHHBAHHAHBHA-BAADAHHBAHHHHHAHHBAHBHHHBBHHH HDAH
Xuah61HHHH-AHHAHAHHHHAHHHBHHHBAHH-HHHBBHAHHA-BHHHH-HBHH- H-HA
Xuah74D-D--DD-D-DBDDD-DD--DD--BBB-DDDDDDDDBBD-DD---BDBD- ----
Xuah82AA-A--AA-H-BHBAH-HH--HB--BBH-HHBBHHAAHBA-HH---BABH- ----
Xuah82BA-A--AA-C-CCCAC-CC--CC--CCC-CCCCCCAACCA-CC---CACC- ----
Xuah82CD-D--DD-D-BDBDD-DD--DB--BBD-DDDBDDDDDBD-DD---BDBD- ----
Xuah97AHHAABAHAHHHHHBAHHHHBHBBHHHCBHAHHHBHHHHAHHHHHHHBAB HHB-
Xwg114CCCAABCCACCCCBCACCCCBBCCBBCCCCCCCCBCCACCCCCCCCCBAC CDD-
Xwg180DDDDDBDDDDDDDDBDDDDDBDBBDDDDBDDDDDBDDDDDDDDDDDDBDB DDD-
Xwg222HAHBHABAHHBAHABABHHAAABHHBH-BBHBAHBHBA-HHHHH-BHHA- A-HB
Xwg364AH-B---H-B-HBHBH-HH--AH--HHH-HBHBHHHAHAH-HB---HBAB- ----
Xwg380BHBBACCBACCBHBCCHBBAAHBBHHABHBHBHHHAHAHCHH-CHHBHBC C---
Xwg516AABHHBHHBHHHAHHAHHHAHHBHHAHHBHHHHHHBHHBAHBHHHBBHAH HBAH
Xwg522AABBAAHBHAHHAAHHHHBAHHHBHAHHBBHHAHBBHHBAHBHHHBBAHB AAHB
Xwg9ABABBAAHHHBDBHHAHHABHBHHBBHHHHHBHHAAHBAAHHHHHBABHH AABB

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