Mapping was done on a population of 240 F5 RILs derived by single seed descent from a cross of Arina x Forno, Swiss winter wheat varieties.
These maps integrate SSRs and RFLPs. Lines were screened with 329 SSRs, and 179 of 186 polymorphic SSRs were used for mapping. RFLP analysis was done initially with 479 probes and seven enzymes. Of 196 polymorphisms, 114 were used for mapping.
Results were confirmed on nullisomic-tetrasomic lines of Chinese Spring.
Linkage analysis was done with MAPMAKER, with an LOD threshold of 4.0 and a maximum distance of 30 cM.