Miyazaki C et al. (2000) A barley linkage map using an F-2 population compared with a map based on female recombination-derived doubled haploid lines Breeding Science 50:241-250.
Miyazaki C et al. (2000) Construction of a barley RFLP linkage map using an F2 population derived from a cross between Ko A and Mokusekko 3 Barley Genetics Newsletter 30:41-43.
An RFLP linkage map was constructed using 120 F2 plants from a cross between Ko A (a Japanese two-rowed malting barley) and Mokusekko 3 (a Chinese six-rowed barley landrace).
Polymorphism was detected using BamHI, BglII, DraI, EcoRV, HindIII and XbaI restriction enzymes.
222 loci were mapped with an average distance of 6.5 cm between markers for a total of 1389 cM, and included 147 genomic DNA RFLPs, 73 cDNA RFLPs, one isozyme (Est1) and one morphological (vrs1) marker. Only three gap regions exceeding 25 cM were detected.
117 RFLP loci were mapped using 91 previously reported probes and 103 loci were mapped using new JBG or JBC probes.