Mapping was performed in recombinant inbred lines derived by single-seed descent (F8) from the cross of the synthetic hexaploid, W7984, x Opata M 85. RI line numbers 1 - 60 and 62 - 115 were used.
Microsatellite loci were integrated into a framework map of 302 RFLPs, using the 'PLACE' command in MAPMAKER 2.0. Marker positions within chromosomes were determined with the 'TRY' and 'RIPPLE' commands. In a few cases, nulli-tetrasomic analysis was used to locate microsatellite loci.
Most (80%) of the microsatellite primers detected a single locus in T. aestivum. Sixty-five microsatellite loci were integrated into the framework map at LOD > 2.5; 214 others were assigned to most-likely intervals.