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GrainGenes Marker Report: AM18

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Locus
am18
Type
Microsatellite
Synonym
Other nameAM18
Map Data
Oat, KxO, 2003
Species
Avena sativa
Probe
AM18
Remark
Marker placement value = 0.00 cM on the Oat-KxO/2003 maps
Marker is unlinked on the Oat-KxO/2003 maps



Probe
AM18
Locus
am18
AM18
Reference
ReferenceLi CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
Remarks
Insert contains repeat (AC)14
Known repeat = 5'-Alu with 69 % identity over 52 base pairs
Among 20 oat cultivars, 1 allele was found.
Type
PCR
SSR
PCR primers
5'CAA TGT CGT CGG TGT GAG TTT
5'TAC GAG TGT GGC ACG AGC
SSR size
270
Amplification Conditions
Average melting temperature (degrees C) = 47
Source Species
Avena sativa
Source Germplasm
Ogle CIav9401
Data Source
Scoles, Graham J.



Locus for Probe
AM18
Type
Microsatellite
Synonym
Other nameam18
Species
Avena sativa
Probe
AM18