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GrainGenes Marker Report: BARE1-5M59n

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Locus
BARE1-5M59n
Type
SSAP
Chromosome
1H
Map
Hordeum-SxM-SSAP-1H82.7
[ Show Nearby Loci ]
Map Data
Barley, Steptoe x Morex, SSAP
Probe
BARE1-5M59
Reference
ReferenceRodriguez M et al. (2006) Integration of retrotransposons-based markers in a linkage map of barley. Molecular Breeding 17:173-184.



Probe for Locus
BARE1-5M59
Locus
BARE1-5M59i
[ Show all 16 ]
Reference
ReferenceLeigh F et al. (2003) Comparison of the utility of barley retrotransposon families for genetic analysis by molecular marker techniques Molecular Genetics and Genomics 269:464-474.
General Remarks
Position of retroposon-based primer in the LTR = 5' terminus + A
MseI primer (KeyGene code) = CTA (M59)
Type
PCR
STS
PCR primers
BARE-1-5980 5' CTAGGGCATAATTCCAACAA 3'
Mse1 primer M59 5' GATGAGTCCTGAGTAACTA 3'
Amplification Conditions
amplified as Vos et al Nucl Acids Res 23: 4407-4419
Source Species
Hordeum vulgare