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GrainGenes Marker Report: BCD1127b

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Locus
BCD1127b
Type
RFLP
Map
Oat-KxO/2003-1311.9
Zea-Ahn-869.25
[ Show Nearby Loci ]
Map Data
Maize, TxCM
Oat, KxO, 2003
Probe
BCD1127
Homology
COQ2_SCHPOe-value: 0.99PUTATIVE PARA-HYDROXYBENZOATE--POLYPRENYLTRANSFERASE, MITOCHONDRIAL PRECURSOR (PHB:POLYPRENYLTRANSFERASE) mitochondrial - fission yeast (Schizosaccharomyces pombe) precursor(ec 2.5.1.-) [Schizosaccharomyces pombe] [Schizosaccharomyces pombe]
Remark
Marker placement value = 2.35 cM on map Oat-KxO/2003-13
Marker is not a framework marker on map Oat-KxO/2003-13
Possible Orthologs
bcd1127
[ Show all 10 ]



Probe for Locus
BCD1127
Locus
BCD1127b
[ Show all 11 ]
Synonym
Please seeBCD828
SynonymHAR35_14757
HAR35_48348
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Bar.LambZAPII
Based on surveys and mapping in Chinese Spring aneuploids and the wheat Synthetic x Opata population, this probe appears to be identical to BCD828, and should be referred to by that name. (Clare Nelson, August 1995)
Type
cDNA
Sequence
BE438744
BCD1127_WHE1H0011
BCD1127_WHE1H0012
Polymorphism
BCD1127 EcoRI
BCD1127 EcoRV
Linkage Group
3L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.7
PCR size
0.7
Clone Vector
pBluSK
pBluSK-
BS-SK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD1127 BROWS autoradiogram
BCD1127/CSpring/EcoRV
BCD1127 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09

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