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GrainGenes Marker Report: BCD351F

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Locus
BCD351F
Type
RFLP
Chromosome
2H
Map
Barley-Consensus2-2H35.3
[ Show all 10 ]
[ Show Nearby Loci ]
QTL with Locus Marker
QGms.StMo-2H
Map Data
Barley consensus 2
[ Show all 10 ]
Probe
BCD351
Homology
BAA83564.1e-value: 1e-16(AP000399) ESTs C97429(C60159),D22427(C11106), AU078031(E31854),D15683(C1084) correspond to a region of the predicted gene.~hypothetical protein [Oryza sativa]
BAA83564.1e-value: 2e-11(AP000399) ESTs C97429(C60159),D22427(C11106), AU078031(E31854),D15683(C1084) correspond to a region of the predicted gene.~hypothetical protein [Oryza sativa]
Reference
ReferenceWenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.
ReferenceRodriguez M et al. (2006) Integration of retrotransposons-based markers in a linkage map of barley. Molecular Breeding 17:173-184.
ReferenceRostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
Data Source
Blake, Tom05.04.19
Remark
Number of populations contributing to the placement of BCD351F in the Barley, Consensus 2006 DArT maps = 1
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Steptoe x Morex
Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Possible Orthologs
BCD351
[ Show all 18 ]



Probe for Locus
BCD351
Locus
BCD351C
[ Show all 31 ]
Synonym
SynonymHAR35_1862
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438985
BE439015
BCD351_WHE05B06.s1
Linkage Group
[b 1H, 2H]
Barley 5 (1H)
4
Barley 7 (5H)
2
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.9
PCR size
0.9
Clone Vector
pBluSK
pBluSK-
pBS
lambda_Zap
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
m13reverse 5'AACAGCTATGACCATG
m13forward 5'GTAAAACGACGGCCACT
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07
Graner, Andreas94.03.08
ATMI92.09
Kleinhofs, Andris96.05.08
Gill, Bikram S.92.09