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GrainGenes Marker Report: BCD402B

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Locus
BCD402B
Type
RFLP
Synonym
SynonymBCD402
Chromosome
2H
4H
Map
Barley-consensus-2003-2H55.2
[ Show all 18 ]
[ Show Nearby Loci ]
QTL with Locus Marker
QAa.StMo-4H.1
QDrm.StMo-4H
QHd.StMo-4H.1
QMe.StMo-4H
Map Data
Barley consensus 2
[ Show all 18 ]
Species
Hordeum vulgare
Probe
BCD402
Reference
ReferenceClose TJ et al. (2009) Development and implementation of high-throughput SNP genotyping in barley. BMC Genomics 10:582.
[ Show all 6 ]
Data Source
Blake, Tom05.04.19
Close, Timothy J.
Langridge, Peter J.
Remark
Number of populations contributing to the placement of BCD402B in the Barley, Consensus 2006 DArT maps = 1
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Steptoe x Morex
Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Steptoe x Morex
Locus in 'Barley, Consensus 2007, SSR' was a BIN marker in 'Barley, Consensus 2006, Marcel'
Possible Orthologs
BCD402
[ Show all 16 ]



Probe for Locus
BCD402
Locus
BCD402
[ Show all 17 ]
Synonym
SynonymHAR35_47809
HAR35_7272
HAR35_7906
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Additional faint insert band of PCR_size 1.6.
Bar.LambZAPII [c]
Bar.LambZAPII
Type
cDNA
Sequence
L44099
BCD402
Polymorphism
BCD402 EcoRI
Linkage Group
[b 4L]
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
3.1
PCR size
3.1
Clone Vector
pBluSK
pBluSK-
BS-SK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
BCD402 BROWS autoradiogram
BCD402 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09