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GrainGenes Marker Report: Bmac0144e

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Locus
Bmac0144e
Type
Microsatellite
Synonym
SynonymBmac144
Chromosome
5H
Map
Hordeum-Consensus2005-SNP-5H76.007
[ Show all 6 ]
[ Show Nearby Loci ]
Map Data
Barley, Abiotic QTL Consensus
[ Show all 6 ]
Species
Hordeum spontaneum
Hordeum vulgare
Probe
Bmac0144
Bmac144
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark
Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum
Insert contains repeat (AT)4(AC)20
Developing laboratory = SCRI (R. Waugh)
Possible Orthologs
Bmac0144
[ Show all 13 ]



Probe for Locus
Bmac0144
Locus
Bmac0144
[ Show all 13 ]
Synonym
Other nameBmac144
Reference
ReferenceHearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Type
SSR
PCR primers
TACGTGTACATACTCTACGATTTG
ACTTATTCTGCATCCTGGGT



Probe for Locus
Bmac144
Locus
Bmac0144a
[ Show all 13 ]
Synonym
Other nameBmac0144
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AT)4(AC)20
Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type
PCR
SSR
PCR primers
5' TACGTGTACATACTCTACGATTTG 3'
5' ACTTATTCTGCATCCTGGGT 3'
SSR size
179
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare