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GrainGenes Marker Report: Bmac0162

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Locus
Bmac0162
Type
Microsatellite
Synonym
SynonymBmac162
Chromosome
7H
Map
Hordeum-Consensus2005-SNP-7H75.52
[ Show all 7 ]
[ Show Nearby Loci ]
Map Data
Barley, Abiotic QTL Consensus
[ Show all 7 ]
Species
Hordeum spontaneum
Hordeum vulgare
Probe
Bmac0162
Bmac162
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark
Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum
Insert contains repeat (AC)15(CT)16
Developing laboratory = SCRI (R. Waugh)
Possible Orthologs
Bmac162



Probe for Locus
Bmac162
Locus
Bmac0162
Bmac162
Synonym
Other nameBmac0162
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)15(CT)16
Quality = 2: Single product, relatively strong stutter bands
Type
PCR
SSR
PCR primers
5' CATGTGTTGAAATCAGTTTTG 3'
5' CCCTCTCTCTCTCTCTCTCTC 3'
SSR size
187
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare



Probe
Bmac0162
Locus
Bmac0162
Synonym
Other nameBmac162
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Type
SSR
PCR primers
CATGTGTTGAAATCAGTTTTG
CCCTCTCTCTCTCTCTCTCTC