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GrainGenes Marker Report: Bmag0603

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Locus
Bmag0603
Type
Microsatellite
Synonym
SynonymBmag603
Chromosome
3H
Map
Barley, B73xCPI-3H46.1
[ Show all 9 ]
[ Show Nearby Loci ]
Map Data
Barley, Abiotic QTL Consensus
[ Show all 9 ]
Species
Hordeum spontaneum
Hordeum vulgare
Probe
Bmag0603
Bmag603
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 6 ]
Possible Orthologs
Bmag603



Probe for Locus
Bmag603
Locus
Bmag0603
Bmag603
Synonym
Other nameBmag0603
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AG)24
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.78
Type
PCR
SSR
PCR primers
5' ATACCATGATACATCACATCG 3'
5' GGGGGTATGTACGACTAACTA 3'
ATACCATGATACATCACATCG
GGGGGTATGTACGACTAACTA
SSR size
120
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare



Probe
Bmag0603
Locus
Bmag0603
Synonym
Other nameBmag603
Reference
ReferenceHayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80.
ReferenceHearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
Type
SSR
PCR primers
ATACCATGATACATCACATCG
GGGGGTATGTACGACTAACTA
Forward ATACCATGATACATCACATCG
Reverse GGGGGTATGTACGACTAACTA