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GrainGenes Marker Report: Bmgtttttt0001

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Locus
Bmgtttttt0001
Type
Microsatellite
Synonym
SynonymBmgtttttt1
Chromosome
6H
Map
Hordeum-Consensus2005-SNP-6H71.859
[ Show all 6 ]
[ Show Nearby Loci ]
Map Data
Barley, Abiotic QTL Consensus
[ Show all 6 ]
Species
Hordeum spontaneum
Hordeum vulgare
Probe
Bmgtttttt0001
Bmgtttttt1
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark
Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum
Insert contains repeat (GTTTTT)5
Polymorphic Information Content (PIC) = 0.5
Developing laboratory = SCRI (R. Waugh)



Probe for Locus
Bmgtttttt1
Locus
Bmgtttttt0001
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (GTTTTT)5
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.5
Type
PCR
SSR
PCR primers
5' ACACCAGAGCCTTGACTCGT 3'
5' AGCAGCAACAACGACAACAC 3'
SSR size
215
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 69C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 68C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 63C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare



Probe
Bmgtttttt0001
Locus
Bmgtttttt0001
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Type
SSR
PCR primers
ACACCAGAGCCTTGACTCGT
AGCAGCAACAACGACAACAC