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GrainGenes Marker Report: CDO420A

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Locus
CDO420A
Type
RFLP
Map
Barley-Consensus2-7H11.1
[ Show all 7 ]
[ Show Nearby Loci ]
Map Data
Barley consensus 2
[ Show all 7 ]
Species
Avena sativa
Avena sativa L.
Probe
CDO420
Data Source
Kleinhofs, Andris
Remark
This marker is a Binlocus in the Hordeum-Bins maps, i.e. a marker mapped in other crosses and located to the Steptoe x Morex bin
Marker placement value = 1.16 cM on map Oat-KxO/2003-17
Marker is not a framework marker on map Oat-KxO/2003-17
Possible Orthologs
CDO420B
[ Show all 8 ]



Probe for Locus
CDO420
Locus
Xcnl.CDO420A
[ Show all 9 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Polymorphism
CDO420 DraI
Linkage Group
[b 1LH, 1SH]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
2.1
PCR size
2.1
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
CDO420 KO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09