Query (optional)   in Class  

GrainGenes Marker Report: CDO455b

[Submit comment/correction]

Locus
CDO455b
Type
RFLP
Map
Oat-OxT-3481.6
Zea-Ahn-3166.6
[ Show Nearby Loci ]
Map Data
Maize, TxCM
Oat, OxT
Probe
CDO455
Possible Orthologs
CDO455
CDO455a
Xcdo455
CDO455B
CDO455A



Probe for Locus
CDO455
Locus
CDO455b
[ Show all 6 ]
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
AA231702
AA231871
Polymorphism
CDO455 EcoRI
Linkage Group
3L
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks (oat)
Source Tissue
etiolated leaf
Insert Size
1.2
PCR size
1.2
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
Clone Authority
Sorrells, Mark E.
Image
CDO455 BROWS autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.