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GrainGenes Marker Report: CDO457a

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Locus
CDO457a
Type
RFLP
Map
Oat-KxO/2003-7_10_28158
Zea-Ahn-50
[ Show Nearby Loci ]
Map Data
Maize, TxCM
Oat, KxO, 2003
Probe
CDO457
Homology
BAB09770.1e-value: 1e-16(AB016890) sugar transporter-like protein [Arabidopsis thaliana]
BAB09770.1e-value: 2e-23(AB016890) sugar transporter-like protein [Arabidopsis thaliana]
Remark
Marker placement value = 2.27 cM on map Oat-KxO/2003-7_10_28
Marker is not a framework marker on map Oat-KxO/2003-7_10_28
Possible Orthologs
CDO457
[ Show all 14 ]



Probe for Locus
CDO457
Locus
CDO457a
[ Show all 14 ]
Synonym
SynonymHAR35_20530
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
OatUni-ZAPXR [c]
Type
cDNA
Sequence
BE439076
[ Show all 6 ]
Polymorphism
CDO457 EcoRI
CDO457 EcoRV
CDO457 HindIII
CDO457 XbaI
Linkage Group
[b 7L]
Insert Enzyme
EcoRI
XhoI
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
Insert Size
1.4
PCR size
1.4
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO457 BROWS autoradiogram
CDO457 KO autoradiogram
CDO457 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09