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GrainGenes Marker Report: EBmac0560d

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Locus
EBmac0560d
Type
Microsatellite
Chromosome
5H
Map
Hordeum-Consensus2007-SSR-5H43.76
Hv-Consensus2006-Marcel-5H43.76
Hv-Integrated2009-5H45.7
Hv-L94/Vada-2006-5H39.68
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Marcel
Barley, Consensus 2007, SSR
Barley, Integrated, Marcel 2009
Barley, L94 x Vada, 2006
Species
Hordeum vulgare
Probe
EBmac0560
EBmac560
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 6 ]
Possible Orthologs
Bmac0560a
[ Show all 7 ]



Probe for Locus
EBmac0560
Locus
EBmac0560
EBmac0560a
EBmac0560b
EBmac0560d
EBmac0560c
Synonym
Other nameEBmac560
Reference
ReferenceHearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Type
SSR
PCR primers
CATATGTGTGCAAGTGTGC
AGATCCATGAGGTTGTGC



Probe for Locus
EBmac560
Locus
EBmac0560a
[ Show all 7 ]
Synonym
Other nameEBmac0560
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)7
Quality = 4: Ladders of bands that are at equal intensity, but may contain scoreable weak products
Type
PCR
SSR
PCR primers
5' CATATGTGTGCAAGTGTGC 3'
5' AGATCCATGAGGTTGTGC 3'
SSR size
171
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare

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