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GrainGenes Marker Report: GBM1022-6H

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Locus
GBM1022-6H
Type
Microsatellite
Map
Hv-Consensus2006-Stein-6H101.7
Hv-DxR-Stein-6H96.98
Hv-IxF-Stein-6H84.1
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Stein
Barley, IxF, Stein2006
Barley, OWB, Stein2006
Probe
GBM1022



Probe for Locus
GBM1022
Locus
GBM1022
GBM1022-6H
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceThiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
General Remarks
Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner
Clone HW07G07u
Type
SSR
EST
PCR primers
available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
AL502381
DNA Library
IPK-HW
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
Clone Location
Graner, Andreas
Data Source
Graner, Andreas
Stein, Nils2006.10