Query (optional)   in Class  

GrainGenes Marker Report: GBM1074

[ Printable Version ]  [ Submit comment/correction ]

Locus
GBM1074
Type
Microsatellite
Chromosome
3H
Map
Hordeum-Consensus2007-SSR-3H20.49
[ Show all 6 ]
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Marcel
[ Show all 6 ]
Species
Hordeum vulgare
Probe
GBM1074
Reference
ReferenceSzucs P et al. (2009) An Integrated Resource for Barley Linkage Map and Malting Quality QTL Alignment The Plant Genome 2:134-140.
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceThiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 7 ]



Probe
GBM1074
Locus
GBM1074
GBM1074-3H
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceThiel T et al. (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.) Theoretical and Applied Genetics 106:411-422.
Remarks
Primer sequences for this SSR are proprietary, those interested should contact Andreas Graner
Clone HK05F10r
Type
SSR
EST
PCR primers
available upon request to authors: graner@ipk-gatersleben.de
Amplification Conditions
3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence
AL500188
DNA Library
IPK-HK
Source Species
Hordeum vulgare
Source Germplasm
Barke (barley)
Clone Location
Graner, Andreas
Data Source
Graner, Andreas
Stein, Nils2006.10



Locus for Probe
GBM1074-3H
Type
Microsatellite
Map
Hv-Consensus2006-Stein-3H33.7
Hv-DxR-Stein-3H33.67
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2006, Stein
Barley, OWB, Stein2006
Probe
GBM1074